Holoenzyme - Study guides, Class notes & Summaries

Question 1 3 / 3 pts True or False: The amino acids serine and threonine both contain sulfur atoms. FALSE Question 2 3 / 3 pts True or False: The following secondary structure shown below is an example of a betaturn. FALSE Question 3 3 / 3 pts True or False. The side chain of phenylalanine is bonded to the backbone nitrogen atom. FALSE Question 4 3 / 3 pts True or False: The name of the molecule that binds to an enzyme is called the holoenzyme. FALSE Question 5 3 / 3 pts

What is gene expression? ~~> dsDNA is decoded to ssRNA/proteins by an mRNA intermediate using A/U/C/GTPs and RNA polymerase Where is the consensus sequence in prokaryotic promoters? ~~> at -10 and -35 upstream of the the transcription start site What recognises the consensus sequences in prokaryotes? ~~> the holoenzyme What is a holoenzyme? ~~> a closed complex with a core enzyme and sigma factor 70 What does the holoenzyme do? ~~> causes the dsDNA to open and allo...

MCBM 1- (6) Enzyme, Kinetics, and Regulation with Complete Questions and Answers 100% CorrectMCBM 1- (6) Enzyme, Kinetics, and Regulation with Complete Questions and Answers 100% CorrectMCBM 1- (6) Enzyme, Kinetics, and Regulation with Complete Questions and Answers 100% CorrectMCBM 1- (6) Enzyme, Kinetics, and Regulation with Complete Questions and Answers 100% CorrectWhat is kcat? What is a holoenzyme? - ANSWER - Kcat=overall rate of an enzyme, catalytic efficiencies. Holoenzyme (active) i...

two parts of holoenzyme (helper to RNA polymerase in transcription in bacteria) correct answers core enzyme and sigma factor (determining correct location for transcription) transcription in bacteria correct answers sigma factors bind to RNA pol core enzyme and direct it to promoter transcription prceeds different sigma factors different genes ways to stop transcription correct answers rho-dependent and rho independent rho dependent correct answers rho protein follows RNA pol and...

Enzymes – Biochemistry Questions and Answers with Verified Solutions What is the primary function of enzymes in biological systems? Enzymes act as catalysts, speeding up chemical reactions without being consumed in the process. How do temperature and pH affect enzyme activity? Enzymes have optimal temperature and pH ranges where they function best; deviations can lead to reduced activity or denaturation. What is the active site of an enzyme? The active site is the specific re...

Exam 2: BIOS 242 / BIOS242 Review (Latest 2024/ 2025 Update) Fundamentals of Microbiology with Lab| Questions and Verified Answers| 100% Correct| Grade A- Chamberlain Q: what are enzymes? Answer: biological catalysts that increase the rate of a chemical reaction Q: is needed for a reaction to occur Answer: activation energy Q: true or false: enzymes lower the activation energy Answer: true Q: enzymes act on reactant molecules known as Ans...

What is kcat? What is a holoenzyme? Correct Answer-Kcat=overall rate of an enzyme, catalytic efficiencies. Holoenzyme (active) is an inactive apoenzyme with non-protein components. (look at the picture) Discuss enzyme-substrate specificity. What is the lock and key vs. induced fit model? Correct Answer-Induced fit: enzyme changes after substrate binds to make it fit more perfectly. Discuss energy implications of enzymes. Does it change the activation energy or free energy of the overal...

What term refers to all of the chemical reactions taking place in a cell? A. Catabolism B. Redox reactions C. Phosphorylation D. Metabolism E. Cellular respiration correct answer: D) Metabolism The breakdown of peptidoglycan to N‐acetylmuramic acid, N‐acetylglucosamine and peptides by a microbe is an example of A. Anabolism B. Catabolism C. Phosphorylation D. Fermentation correct answer: B) Catabolism Enzymes are typically A. Broken down in reactions that requires energy i...

BMS 503 Exam 3 Questions with Correct Answers 100% Verified 2024/2025 What are the 3 major steps of replication, transcription, and translation? - Correct Answer initiation, elongation, termination Bacterial RNA Polymerase - Correct Answer 5 proteins in the core complex that are holoenzymes; 2 alpha: interacts with regulatory proteins; beta: catalytic activity; beta prime: involved in binding and staying bound to DNA; w: stabilizes the complex Sigma Factor - Correct Answer controls the bi...

Eukaryotic promoters have a basal region which can contain a TATA box, initiator (Lnr), BRE (TFIIV recognition element) and what other element? ~~> Downstream core promoter (DPE) In prokaryotes RNA polymerase requires what co-factor? ~~> 70 sigma factor Forms the holoenzyme The promoter is formed of two hexameric sequences at what positions? ~~> -35 and -10 The amount of product transcribed is dependant on how close the promoter sequences are to what? ~~> Consensus s...