100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
CMB2001 Questions and Answer | Latest Update 2024/2025 $13.49   Add to cart

Exam (elaborations)

CMB2001 Questions and Answer | Latest Update 2024/2025

 7 views  0 purchase
  • Course
  • CMB2001
  • Institution
  • CMB2001

What is gene expression? ~~> dsDNA is decoded to ssRNA/proteins by an mRNA intermediate using A/U/C/GTPs and RNA polymerase Where is the consensus sequence in prokaryotic promoters? ~~> at -10 and -35 upstream of the the transcription start site What recognises the consensus sequences...

[Show more]

Preview 4 out of 67  pages

  • September 15, 2024
  • 67
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • CMB2001
  • CMB2001
avatar-seller
ExamArsenal
1 | P a g e | © copyright 2024/2025 | Grade A+




CMB2001 Questions and Answer |
Latest Update 2024/2025
What is gene expression?


✓ ~~> dsDNA is decoded to ssRNA/proteins by an mRNA intermediate

using A/U/C/GTPs and RNA polymerase




Where is the consensus sequence in prokaryotic promoters?


✓ ~~> at -10 and -35 upstream of the the transcription start site




What recognises the consensus sequences in prokaryotes?


✓ ~~> the holoenzyme




What is a holoenzyme?


✓ ~~> a closed complex with a core enzyme and sigma factor 70




What does the holoenzyme do?


✓ ~~> causes the dsDNA to open and allows for elongation to happen,

after 10 bases are added the sigma factor is released




Master01 | September, 2024/2025 | Latest update

, 2 | P a g e | © copyright 2024/2025 | Grade A+


What does eukaryotic DNA have near the transcription start site?


✓ ~~> core/basal regions




What is a possible core/basal region?


✓ ~~> TATA box




Where is a TATA box located?


✓ ~~> -26/-31




What is a possible core/basal region?


✓ ~~> initiator (INR)




Where is the initiator located?


✓ ~~> -2/+4




What is a possible core/basal region?


✓ ~~> downstream core promoter (DPE)




Where is the downstream core promoter located?




Master01 | September, 2024/2025 | Latest update

, 3 | P a g e | © copyright 2024/2025 | Grade A+


✓ ~~> +30




What is a possible core/basal region?


✓ ~~> TFIIB recognition element (BRE)




Where is the TFIIB recognition element located?


✓ ~~> -37/-32




What is a possible core/basal region?


✓ ~~> CpG islands




What are CpG islands?


✓ ~~> DNA regions with many cytosine bases adjacent to guanine

bases




What makes CpG islands different?


✓ ~~> normally the C residues next to G are methylated to 5-methyl C

but in the islands they are not




What effect does methylation have on gene transcription?



Master01 | September, 2024/2025 | Latest update

, 4 | P a g e | © copyright 2024/2025 | Grade A+


✓ ~~> can either increase or decrease transcription of genes

depending on which amino acids are methylated, and how many

methyl groups are attached




What does eukaryotic DNA have upstream of the transcription start site?


✓ ~~> upstream regulatory regions




Whats an examples of upstream regulatory regions?


✓ ~~> CAAT box which is an UAS (upstream activating sequences)




Whats an examples of upstream regulatory regions?


✓ ~~> GC box (UAS)




What are UAS/URS?


✓ ~~> upstream activating/repressor sequences without activator

sequences the formation of PIC is ineffective and leads to poor

transcription




A way to identify promoter elements?




Master01 | September, 2024/2025 | Latest update

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller ExamArsenal. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $13.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

82191 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$13.49
  • (0)
  Add to cart