Plasmid Study guides, Class notes & Summaries

CMMB 343 Final Question and answers already passed CMMB 343 Final How does phi6 penetrate the host cell? - correct answer - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis - once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm Which protein is the plug at the end of the tail tube in T4, preventing DNA f...

MICROBIOLOGY FINAL EXAM PRACTICE 2024| 300 QUESTIONS AND CORRECT ANSWERS Fixed phagocytes of the epidermis are called: Dendritic cells Cestodes are: Tapeworms Candidiasis is also known as: Moniliasis Progressive dyspnea resulting from the alveolocapillary blockage caused by asexually reproducing trophozoites that fail to evoke a tissue macrophage response describe the pathogenicity of: Pneumocystosis An extrachromosomal genetic element: Plasmid

Creating DNA Libraries -Collections of cloned DNA fragments from a particular organism contained in plasmid vectors within host bacteria -Screen library to pick out different genes of interest Genomic DNA Libraries -Used to locate fragment with DNA of interest -Chromosomal DNA from tissue of interest is isolated and digested using a restriction enzyme (sticky/blunt ends) -Vector also digested -DNA ligase used to ligate chromosomal DNA fragment inside vector DNA (plasmids) -Recombinant ...

CMMB 343 Final Question and answers 100% correct 2024How does phi6 penetrate the host cell? - correct answer - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis - once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm Which protein is the plug at the end of the tail tube in T4, preventing DNA from leaving the ta...

Cyclic AMP A secondary messenger important in many biological processes. It is a derivative of ATP and is used for intracellular signal transduction in many organisms. Which is a secondary messenger? Cyclic AMP Plasmid Small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Naturally exist in bacterial cells, but occur in some eukaryotes. Carry genes (act as vehicle) that provide bacteria with genetic advantages, such as antibiotic resistance. Wh...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

MCB Exam 3 Questions & Answers 2023/2024 What is meant by recombinant DNA? - ANSWER-DNA w/fragments from 2+ sources Name the tools used to make a recombinant DNA. (mechanisms of gene cloning) - ANSWER-1) Isolate plasmid DNA from bacterial cell 2) Isolate DNA of interest 3) Insert gene of interest into plasmid DNA 4) Insert now recombinant DNA into bacterium 5) Culture bacteria to make copies Restriction enzymes - ANSWER-endonuclease that cuts DNA at specific sites Cloning vecto...

MCB4304 Ch 4 Exam 1 Questions & Answers 2024/2025 Ch 4 Which of the following enzymes cannot catalyze the formation of a phosphodiester bond? a. Endonuclease b. RNA polymerase c. DNA polymerase d. Ligase e. None of the above - ANSWERSE Ch. 4 Select the component that gives a plasmid its host range. a. Tetracycline resistance b. Ampicillin resistance c. EcoRI site d. Origin of replication e. ligase - ANSWERSD Ch. 4 A molecular biologist is able to select recombinant c...

A bacterial cell is found to be motile and resistant to high temperature and phagocytosis. This cell probably has: A. Flagella, pili, plasmid B. Flagella, pili, capsule C. Endospore, capsule, ribosome D. Endospore, flagella, capsule - ️️D. Endospore, flagella, capsule A bacterial cell that prefers the presence of oxygen but can survive without it is called a/an A. facultative anaerobe B. obligate aerobe C. aerotolerant anaerobe D. strict anaerobe E. microaerophile - ️️A. facult...

Microbiology Exam 4 (Practice Questions) & Answers 2024 All of the following are generally used in vaccines EXCEPT... a. toxoids b. parts of bacterial cells c. live, attenuated viruses d. inactivated viruses e. antibodies - ANSWER-e. antibodies A reaction between an antibody and soluble antigen-forming lattices is called a(n)... a. agglutination reaction b. complement fixation c. immunofluorescence d. neutralization reaction e. precipitation reaction. - ANSWER-e. precipita...