Plasmid - Study guides, Class notes & Summaries

What is antibiotic selection? Correct Answer-Survival of a population of antibiotic resistant cells within a larger population of antibiotic sensitive cells. Where can antibiotic selection occur? Correct Answer-Anywhere where there are antibiotics and bacteria present. The best-surviving bacteria will be selected for. Name a common use for antibiotic selection in microbiological research. Correct Answer-To identify and isolate cells that have piece of DNA of interest such as a particular p...

WGU C652 Exam Review (Latest 2024/ 2025 Update) Heredity and Genetics | Questions and Verified Answers| 100% Correct| Grade A Q: chemical modifications of nucleotides Answer: Chemical mutagens can modify bases in DNA and alter their base-pairing properties Q: tetraploidy Answer: containing four homologous sets of chromosomes Q: trisomy Answer: a condition in which an extra copy of a chromosome is present in the cell nuclei, causing developmental abnormalities...

loop of Henle correct answers where Na+ is reabsorbed from the filtrate moving through the nephron This is ultimately the mechanism whereby the kidneys concentrate urine - section of the nephron tubule that conserves water and minimizes the volume of urine action potentials result in correct answers an increased permeability of the plasma membrane to sodium - Na+ in causes depolarization hyperglycemia promotes cellular dehydration because correct answers glucose molecules raise the osmo...

BIOE 206 Exam 1 UPDATED ACTUAL Questions and CORRECT Answers Plasmid DNA purification steps - CORRECT ANSWER- 1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt ...

AP Biology Semester 1 Review Latest Update Graded A+ 3 phases of transcription initiation, elongation, termination 3 steps of protein synthesis transcription, RNA processing, translation 5' cap The end of a pre-mRNA molecule modified by the addition of a cap A site One of a ribosome's three binding sites for tRNA during translation. Holds the tRNA carrying the next amino acid to be added to the polypeptide chain. acid any substance that dissociates in water to increase the c...

Vertical gene transfer is the process of genes being passed down from parent to offspring Correct Answer-True When bacteria take up naked genetic material from the surrounding environment, this is called Correct Answer-transformation When one bacterium transfers a copy of its plasmid to another bacterium, this is called Correct Answer-conjugation Transformation. transduction. and conjugation are all examples of Correct Answer-horizontal gene transfer What is the name of the process o...

Biology 1402- Test 1 Questions and Answers Already Passed A community plus its physical environment is a/an ________. A) thallus B) ecosystem C) population D) plasmid ecosystem The ultimate source of energy for nearly all life on Earth is the sun; plants and certain other organisms convert solar energy into chemical energy by the process of _________. A) the citric acid cycle B) cellular respiration C) photosynthesis D) glycolysis photosynthesis ___________ includes chan...

CMMB 343 Exam Study Guide with 100% Correct Answers | Verified | Latest 2024 Version How does phi6 penetrate the host cell? - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis - once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm Which protein is the plug at the end of the tail tube in T4, preventing DNA from...

MIP 300 Exam 4Horizontal gene transfer - correct answer The sharing of genes between different bacterial strains and species through multiple different mechanisms Horizontal gene transfer is responsible for ____________ than even spontaneous mutations - correct answer More genetic diversity Recombination - correct answer Any genes from one cell passed to a recipient, DNA combined with recipient DNA forming a new hybrid DNA 3 mechanisms of prokaryotic horizontal gene transfer, 3 ways pro...

Core Promoter Elements ~~> TATA box = repetitive sequence of thymine and adenine Initiator (INR) = sequence of pyramidines like thymine and cytosine DPE = downstream promoter elements found along the DNA Common to have >1 promoter but not all of them CpG islands ~~> Sequences high in CG frequency. They are able to escape methylation which would lead to gene silencing. Demethylation would lead to over expression of genes. Regulatory Promoter Elements ~~> UAS and URS f...