MCB4304 Ch 4 Exam 1 Questions & Answers 2024/2025
Ch 4
Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?
a. Endonuclease
b. RNA polymerase
c. DNA polymerase
d. Ligase
e. None of the above - ANSWERSE
Ch. 4
Select the component that gives a plasmi...
Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?
a. Endonuclease
b. RNA polymerase
c. DNA polymerase
d. Ligase
e. None of the above - ANSWERSE
Ch. 4
Select the component that gives a plasmid its host range.
a. Tetracycline resistance
b. Ampicillin resistance
c. EcoRI site
d. Origin of replication
e. ligase - ANSWERSD
Ch. 4
A molecular biologist is able to select recombinant colonies from a bacterial culture plate based on the
color of the colonies. Which of the following is a correct statement?
a. Colonies that contain recombinant plasmids are red
b. Colonies that contain recombinant plasmids are blue due to the disruption of the lacZ gene insert
c. Colonies that contain recombinants are clear/white due to the disruption of the lacZ gene by the insert
d. Colonies are blue due to the disruption of the ampicillin gene
, e. All of these choices are correct - ANSWERSC
Ch. 4
One approach to prevent religation of the ends of a restricted plasmid DNA is to:
a. store the plasmid in DNAse
b. store the plasmid in RNAse
c. treat the plasmid with alkaline phosphatase to remove from the 'sticky ends'
d. treat the plasmid with a protease to remove all proteins
e. treat the plasmid with BamHI to create gaps - ANSWERSC
Ch. 4
A recombinant plasmid was constructed with several restriction enzyme sites and an ampicillin
resistance marker. This recombinant was used to transform a host bacteria that also has the amp
resistant gene. Which of the following statement is true regarding this experiment?
a. All transformed bacteria will grow on a plate containing ampicillin
b. All untransformed bacteria will grow on a plate containing ampicillin
c. It will be difficult to screen for a colony containing a plasmid
d. A bacterial host that is ampicillin-sensitive should be used for the screening
e. All of the choices are true - ANSWERSE
Ch. 4
What is the RACE technique?
a. Screening a genomic library
b. Extending incomplete cDNA sequences
c. Generating polynucleotide probes
d. Rapid amplification of genomic DNA
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