Histotechnology - Study guides, Class notes & Summaries

Histotechnology--Nuclear & Cytoplasmic Staining c. nuclei *hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei Harris hematoxylin is used on tissue sections to stain: a. fat b. glycogen c. nuclei d. cytoplasm b. oxidation *can be done naturally (atmospheric oxygen), or with oxidizing agents Ripening of hematoxylin is a process of: a. hydrolysis b. oxidation c. mordanting d...

Select the procedure suitable for demonstrating secretory granules in a carcinoid tumor: A. Wilder reticulum B. PAS-aniline blue C. Snook D. Sevier-Munger D. Sevier-Munger The Sevier-Munger technique is an excellent argyrophil procedure for demonstrating carcinoid tumors. It is similar to, but shorter than, the Grimelius. In Bodian's method for nerve fibers and neurofibrils, Protargol impregnates both neurofribrils and connective tissue. Subsequently, connective tissue is rendere...

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...

ASCP Practice Questions – Histotechnology Exam Questions and Answers (Latest Update 2025) 100% Verified Answers

What method is used to stabilize proteins making it resistant to further damage? - correct answers Fixation Name two methods of fixation? - correct answers Chemical reaction (formaldehyde) and physical (heat or dessication) What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** - correct answers bacteria or mold What factor causes autolysis if tissue is not fixed? **** - correct answers enzyme attack What are four functions of fixatives? - correct answers 1...

Histotechnology HTL Exam - Comprehensive Review questions with correct answers

Periodic Acid Schiff stain demonstrates CORRECT ANSWER-Polysaccharides, neutral mucosubstances, and basement membranes Periodic acid and Chromic acid oxidise carbohydrates to CORRECT ANSWER-aldehydes What acid causes loss of quinoid structure in basic fuchsin? CORRECT ANSWER-Sulfurous acid The control of PAS is CORRECT ANSWER-Kidney The control for PAS-D is CORRECT ANSWER-Liver - one section "with" and one section "without" Alcian blue pH 2.5 demonstrates CORRECT ANSWER-Acid m...

fixitive used for specimens - correct answers 10% neutral buffered Formalin Marking Margins - correct answers - to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station - correct answers phenolic disenfectant or gluteroldehyde how long is specimens stored for - correct answers 1-3 months some legal cases kept indefinitly Why is fresh tissue not stored? - correct answ...

Histotechnology - FixationThe primary purpose of fixation is to: a. stop enzymatic action b. remove the water from the tissue c. provide support for the tissue d. autolyze the tissue - correct answers a Which of the following fixatives contains picric acid, formalin, and acetic acid? a. Zenker b. Helly c. Bouin d. Zamboni - correct answers c The volume of fixative should exceed the volume of the tissue by: a. 1 time to 2 times b. 5 times to 10 times c. 15 times to 20 ...

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...