Histotechnology - Study guides, Class notes & Summaries

Histotechnology Final 1st Quarter T/F To be characterized as a profession, considerable specialized training must be required. - correct answers T Histology is the study of - correct answers tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. - correct answers T T/F Accessioning is giving a specimen a common number, one that is not unique to the patient. - correct answers f T/F The tasks performed by the histot...

Histotechnology Exam 1 Questions (chapter 1) Questions and Answers (Latest Update 2025) 100% Verified Answers

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

HISTOTECHNOLOGY TEST 2 YEAR 3What stains are used for the detection of acid fast bacteria? - correct answers Kinyoun, Ziel-Neelson, Fite, Auramine-rhodamine fluorescence List the types of pathogenic microorganisms - correct answers Bacteria, Fungi, Viruses, Protozoans, Prions Which microorganism requires extended time in formic acid to be inactivated? - correct answers prions What clues may be present on an H and E to indicate the need for microorganism staining? - correct answers incre...

PART II : HISTOTECHNOLOGY HISTOTECHNOLOGY - correct answers Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence of absence of a disease a.) Histology b.) Histopathology c.) Histotechnology d.) Histotechnique AOTA - correct answers INSTRUMENTATION: Laboratory maintain a current file of every piece of equipment in the laboratory including: I. Name, manufacturer, model number, serial number II. Record of prevent...

Histotechnology--Nuclear & Cytoplasmic Stainingc. nuclei *hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei Harris hematoxylin is used on tissue sections to stain: a. fat b. glycogen c. nuclei d. cytoplasm b. oxidation *can be done naturally (atmospheric oxygen), or with oxidizing agents Ripening of hematoxylin is a process of: a. hydrolysis b. oxidation c. mordanting d. ...

Histotechnology Practicum: Test 1 Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most...

What thickness are paraffin sections routinely cut? - Answer 4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? - Answer water bath that is too hot may distort tissue morphology Warm enough to relieve compression Cool enough so the wax does not melt What thickness are paraffin sections cut for the Luxol Fast Blue procedure? - Answer 10 - 15 um Why are paraffin blocks put on ice prior to cu...

Histotechnology test 2 What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 de...

HISTOTECHNOLOGYArt and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. Histotechnology Used to view slides not seen by the naked eye microscope Used by pathologist to view slides to identify a disease or process. microscope Used by histotechnologist to view slides FOR QUALITY CONTROL microscope Basic instrument used in microtomy (the process by which processed tissue, most common...