Histotechnology - Study guides, Class notes & Summaries

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

HISTOTECHNOLOGY TEST 2 YEAR 3What stains are used for the detection of acid fast bacteria? - correct answers Kinyoun, Ziel-Neelson, Fite, Auramine-rhodamine fluorescence List the types of pathogenic microorganisms - correct answers Bacteria, Fungi, Viruses, Protozoans, Prions Which microorganism requires extended time in formic acid to be inactivated? - correct answers prions What clues may be present on an H and E to indicate the need for microorganism staining? - correct answers incre...

PART II : HISTOTECHNOLOGY HISTOTECHNOLOGY - correct answers Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence of absence of a disease a.) Histology b.) Histopathology c.) Histotechnology d.) Histotechnique AOTA - correct answers INSTRUMENTATION: Laboratory maintain a current file of every piece of equipment in the laboratory including: I. Name, manufacturer, model number, serial number II. Record of prevent...

Histotechnology Practicum: Test 1 Biopsy or Gross Specimen: small piece of tissue and derived from simple-natured procedure - correct answers biopsy biopsy or Gross specimen: whole organ and typically derived from surgeries - correct answers gross specimen What are the 10 steps in the pathology lab workflow? - correct answers 1. fixation 2. accession 3. grossing 4. continue fixation 5. dehydration 6. clearing 7. infiltration 8. blocking 9. section 10. staining what is the most...

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...

Histotechnology test 2 What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 de...

HISTOTECHNOLOGYArt and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. Histotechnology Used to view slides not seen by the naked eye microscope Used by pathologist to view slides to identify a disease or process. microscope Used by histotechnologist to view slides FOR QUALITY CONTROL microscope Basic instrument used in microtomy (the process by which processed tissue, most common...

Histotechnology--Nuclear & Cytoplasmic Staining c. nuclei *hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei Harris hematoxylin is used on tissue sections to stain: a. fat b. glycogen c. nuclei d. cytoplasm b. oxidation *can be done naturally (atmospheric oxygen), or with oxidizing agents Ripening of hematoxylin is a process of: a. hydrolysis b. oxidation c. mordanting d...

Select the procedure suitable for demonstrating secretory granules in a carcinoid tumor: A. Wilder reticulum B. PAS-aniline blue C. Snook D. Sevier-Munger D. Sevier-Munger The Sevier-Munger technique is an excellent argyrophil procedure for demonstrating carcinoid tumors. It is similar to, but shorter than, the Grimelius. In Bodian's method for nerve fibers and neurofibrils, Protargol impregnates both neurofribrils and connective tissue. Subsequently, connective tissue is rendere...

What thickness are paraffin sections routinely cut? - Answer 4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? - Answer water bath that is too hot may distort tissue morphology Warm enough to relieve compression Cool enough so the wax does not melt What thickness are paraffin sections cut for the Luxol Fast Blue procedure? - Answer 10 - 15 um Why are paraffin blocks put on ice prior to cu...