Microarray - Study guides, Class notes & Summaries

List tools used in genetic engineering and recombinant DNA technology - Some important Tools used are in silico analysis, DNA microarray analysis, gel electrophoresis, PCR and RFLP analysis Describe/ explain and define PCR - Technique that quickly and easily makes copies of even very small amounts of DNA Describe/ explain and define: Reverse Transcriptase - A RNA dependent DNA polymerase that synthesizes DNA based on an RNA template . Turns single stranded RNA into double stranded DNA

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...

Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune system. Some vaccinations are comprised of a weakened live virus and are not recommended for those who are immunocompromised. What strategies can help protect the immunocompromised individuals in a community? Choose all that apply. (A) Achieve high vaccination rates to provide herd immunity. (B) Vaccinate them even if they are immunocompromised. (C) Create an alternative non-live vaccine. (C)...

Mrcpsych Mentor Statistics Part 1 Exam Questions & Answers 2024 Reporting Guideline: CONSORT - ANSWER-Consolidated Standards of Reporting Trials Reporting Guideline: TREND - ANSWER-Transparent Reporting of Evaluations with Non-Randomised Designs Reporting Guideline: QUORUM - ANSWER-Quality of Reporting of Meta-Analyses Used to examine heterogeneity in a meta-analysis often as a supplement to a forest plot - ANSWER-Galbraith Plot (aka Radial Plot) Reporting Guideline: PRISMA - AN...

4. Which of the following sequence is the correct one for replicating DNA? Not all the steps are included. I. RNA Primers anneal to the DNA template II. ssDNA binding proteins bind to stabilize single strand DNA after helicase unwinds it. III. RNA primers are removed and new DNA strand is made by DNA polymerase IIV. DNA ligase connects the DNA fragments V. Complementary copy of DNA is made by DNA polymerase III A) I, II, III, IV, V B) II, III, IV, I, V C) V, I, III, II, IV D) II, I, V, ...

MCB 150 Final Exam Review with Questions and Answers 2024 Update MCB 150 Final Exam Review with Questions and Answers 2024 Update Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune system. Some vaccinations are comprised of a weakened live virus and are not recommended for those who are immunocompromised. What strategies can help protect the immunocompromised individuals in a community? Choose all that apply. What experimental question(s) can...

DNA SEQUENCING AND MICROARRAY EXAM QUESTIONS AND ANSWERS GRADED A+ DNA SQUENCING - Answers It determines the exact sequence of nucleotides/ bases in a DNA molecule ACTG (nucleotides) - Answers Encode biological informations sequence of DNA - Answers establishing the__________ is key to understanding the functions of genes and other parts of the genome -detection of mutations -typing microorganisms -identifying human haplotyles -designating polymorphisms - Answers Uses of DNA Sequencing ...

Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune system. Some vaccinations are comprised of a weakened live virus and are not recommended for those who are immunocompromised. What strategies can help protect the immunocompromised individuals in a community? Choose all that apply. (A) Achieve high vaccination rates to provide herd immunity. (B) Vaccinate them even if they are immunocompromised. (C) Create an alternative non-live vaccine. (C)...

Tools used in biotechnology and genetic engineering - Answer-In Silico analysis, DNA microarray analysis, gel electrophoresis, PCR, and RFLP analysis. PCR - Answer-*Polymerase Chain Reaction* technique for quickly and easily making many copies of even a very small amount of DNA Reverse Transcriptase - Answer-enzyme that turns single-stranded RNA into double-stranded DNA Complementary DNA (cDNA) - Answer-DNA produced from an RNA template using reverse transcriptase. often used to clone ...

SNV Correct Answer: Any sequence change that affects one base of genome SNPs are common ones Promoter Correct Answer: specific region of a gene where RNA polymerase can bind and begin transcription Nucleosome Correct Answer: 150 bps of DNA around core proteins Stop Codons Correct Answer: UAA, UAG, UGA Karyotype Analysis Correct Answer: Usually done at metaphase Detects trisomy, deletions, duplications, translocations Confirming test FISH Analysis Correct Answer: Fluores...