Microarray - Study guides, Class notes & Summaries

What is the goal of PCR? Correct Answer-To copy and amplify a DNA sequence. What are the requirements of PCR? Correct Answer-Template, primers, deoxyribosenucelotides, DNA polymerase, buffer What is are the general steps of PCR? Correct Answer- What is the purpose of Microarray/chip based hybridization? How do we accomplish this? Correct Answer-We want to get a GLOBAL profile of gene expression. We do this by determining the number of genes expressed in a cell and with how much abundanc...

The cDNA prepared from cancer cells is labeled red and the cDNA prepared from normal tissue is labeled green. A DNA microarray experiment is performed, and it indicates that there are increased levels of expression of the gene dbl in the cancer cells compared to the normal tissue. What result indicated this? A) The spot corresponding to dbl is yellow. B) The spot corresponding to dbl is red. C) The spot corresponding to dbl is green. D) The spot corresponding to dbl is black. E) None of ...

FIN 435 Case Study his case written assignment is on Arcadian Microarray Technologies, In. (Case 44). Report Requirements: · Cover sheet with case name, date, team number and team members; · One or two page written report analyzing questions given; and · Exhibit with any financials, ratios, charts/graphs that you address in your report. Your analysis should cover the following concerns: 1. 3. 1.. Regarding the cash flow forecasts in case Exhibit 5, at what point in the future would yo...

1. Which of these technologies is NOT used to determine the NOTEL (No Observed Transcriptional Effect Level)? a) Protein microarray b) qPCR (RT-PCR) c) SAGE d) cDNA microarray Ans: a) Protein microarray 2. You want a bioindicator to detect the presence of heavy metals in, and around a creek. You would naturally chose: a) a fish b) Daphnia c) a water plant d) It doesn't really matter, because all of the species above may indicate the presence of heavy metals through specific patt...

PNB 2XC3 Final Exam 2024 Real Update Solved 100% whats the purpose? what assumptions are made?, what data supports this?, what concepts and theories are used?, what conclusions can be drawn?, what are the implications and consequences? Answer- Elements of analytical thinking one should use for informed decisions Clarity Accuracy Precision Relevance Depth Breadth Logic Significance Fairness Answer- Intellectual Standards can i understand it? Answer- clarity is it err...

BCHM 270 EXAM QUESTIONS WITH CORRECT ANSWERS A physician would like to evaluate the elevated amounts of a gene being expressed in two different types of tumor cells in order to develop a personalized medicine. Which of the following techniques would be the most appropriate for this purpose? A. Western Blot B. Southern Blot C. Northern Blot D. Eastern Blot E. enzymatic assay - Answer-C A restriction enzyme can be used for which techniques? A. Southern blots and cloning. B. western blo...

Children receiving chemotherapy or bone marrow transplants to treat cancer have a weakened immune system. Some vaccinations are comprised of a weakened live virus and are not recommended for those who are immunocompromised. What strategies can help protect the immunocompromised individuals in a community? Choose all that apply. (A) Achieve high vaccination rates to provide herd immunity. (B) Vaccinate them even if they are immunocompromised. (C) Create an alternative non-live vaccine. (C)...

FGP Mid Semester Test Exam Questions With 100% Correct Answers. 1. Which of these technologies is NOT used to determine the NOTEL (No Observed Transcriptional Effect Level)? a) Protein microarray b) qPCR (RT-PCR) c) SAGE d) cDNA microarray - answera) Protein microarray 2. You want a bioindicator to detect the presence of heavy metals in, and around a creek. You would naturally chose: a) a fish b) Daphnia c) a water plant d) It doesn't really matter, because all of the species above...

SNV Correct Answer: Any sequence change that affects one base of genome SNPs are common ones Promoter Correct Answer: specific region of a gene where RNA polymerase can bind and begin transcription Nucleosome Correct Answer: 150 bps of DNA around core proteins Stop Codons Correct Answer: UAA, UAG, UGA Karyotype Analysis Correct Answer: Usually done at metaphase Detects trisomy, deletions, duplications, translocations Confirming test FISH Analysis Correct Answer: Fluores...

List tools used in genetic engineering and recombinant DNA technology - Some important Tools used are in silico analysis, DNA microarray analysis, gel electrophoresis, PCR and RFLP analysis Describe/ explain and define PCR - Technique that quickly and easily makes copies of even very small amounts of DNA Describe/ explain and define: Reverse Transcriptase - A RNA dependent DNA polymerase that synthesizes DNA based on an RNA template . Turns single stranded RNA into double stranded DNA