Streak plate method - Study guides, Class notes & Summaries
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BIO 250L Lab 2 Culturing & Aseptic Technique Straighterline 2023
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1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. 
What is one experimental way you can test your practices to confirm that you 
are using proper aseptic technique?This could be done by having a control agar 
plate where sterile loop was used to streak the agar. If it grows bacterial colonies after 
some time, then aseptic technique was not successful. 
2. In a laboratory setting, what are three ways you can properly sterilize culturing 
equipment? 
a. Dry hea...
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BIOD 171 Module 4 Quiz with Verified Answers |Latest 2024/2025
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What are the requirements of a fastidious microbe? A fastidious microbe is an organism with complex growth requirements and will not grow without then. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. 
 
What is the name of the process of spreading a bacterial culture onto a petri dish? Plating. Plating microbes can be done using a sterile loop, a sterile swab, or a sterilized wire loop. Each device is simpl...
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures 
 
Lab 2: Techniques for Isolation of Pure Cultures 
 
 
Learning Objectives: 
 
 
Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
Learn the streak plate method 
 
 
 
 
Introduction: 
 
 
Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture ...
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BIOS 242 Lab 1: Culture Transfer Techniques
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Lab 2: Techniques for Isolation of Pure Cultures 
Learning Objectives: 
 Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
 Learn the streak plate method 
VIDEO: 
 Making a streak Plate (Bio-Rad Laboratories) 

Introduction: 
Microorganisms exist in nature as mixed populations of different species. In order to study them and 
observe their characteristics it is first necessary to obtain pure cultures (a culture containing only one 
species of an o...
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PRACTICAL: UConn MCB 2610 Final Lab Practical Exam 100% Solved
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PRACTICAL: UConn MCB 2610 Final Lab Practical Exam 
100% Solved 
The ________ method is a quick and easy way to isolate bacteria. CORRECT ANS-Streak 
plate 
How many times do you get culture from the tube while performing a t-streak? CORRECT 
ANS-Once 
Who do you contact to reschedule a lab? CORRECT ANS-Gino
Too much month left at the end of the money?
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FINAL EXAM: MCB 2610 Lab final Exam with Certified Solutions
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FINAL EXAM: MCB 2610 Lab final Exam with Certified 
Solutions 
The ______ _______ method is a quick and easy way to isolate bacteria CORRECT ANSstreak plate 
In what orientation do you incubate the plates after they have been streaked? CORRECT 
ANS-agar half bottom
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Micro Quiz 2 GCU WITH COMPLETE SOLUTION
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Micro Quiz 2 GCU WITH COMPLETE SOLUTION 
 
 
Agar 
a gel-like polysaccharide compound used for culturing microbes; extracted from certain red algae 
 
 
 
 
 
 
Bacterial reproduction known as 
Binary Fission 
 
 
 
 
 
 
Biofilms 
Most microbes live in polysaccharide-encased communities termed 
 
 
 
 
 
 
Define Pure culture and a colony 
All the bacterial cells that result from the replication of a single original bacterial organism 
 
 
 
 
 
 
Method for isolating bacteria 
Streak-plate me...
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Microbiology Lab 2 Exam Questions And Answers
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combinations of multiple microbial species in a lab setting - ANS mixed culture 
 
cultures that contain only one species of microbe 
 
are absolutely required when characterizing cell structure, biochemistry, physiology, growth parameters, genetics, or other features - ANS pure culture 
 
pure cultures can become ________ __________ when unwanted microbes are introduced through sloppy aseptic technique, inadequate sterilization, improper storage, or mixed microbial stocks - A...
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Mic 205 exam 2 questions with correct answers
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Growth CORRECT ANSWER Refers to an increase in cell number 
 
Discrete colony CORRECT ANSWER Aggregation of cells visible on surface of solid media 
 
Dispersed cells CORRECT ANSWER Single cells suspended in liquid medium 
 
Complex biofilm CORRECT ANSWER Community of one or more species of bacteria 
 
Clinical specimens CORRECT ANSWER Human materials examined or tested for presence of bacteria and other microbes 
 
Considerations of clinical specimens CORRECT ANSWER Technique, timely delivery, ...
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Portage Learning BIOD 171 Lab Exams 1-8 Lecture Exams 2022
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Portage Learning 
BIOD 171 
Lab Exams 1-8 
Lecture Exams 2022 
Lecture Exams 
1. What three elements are used in an autoclave to sterilize equipment? 
heat, pressure, and steam 
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 
125°C 
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to 
best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. 
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