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Exam (elaborations)

FINAL EXAM: MCB 2610 Lab final Exam with Certified Solutions

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  • Course
  • MCB 2610
  • Institution
  • MCB 2610

FINAL EXAM: MCB 2610 Lab final Exam with Certified Solutions The ______ _______ method is a quick and easy way to isolate bacteria CORRECT ANSstreak plate In what orientation do you incubate the plates after they have been streaked? CORRECT ANS-agar half bottom

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  • September 3, 2024
  • 19
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MCB 2610
  • MCB 2610
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FINAL EXAM: MCB 2610 Lab final Exam with Certified
Solutions
The ______ _______ method is a quick and easy way to isolate bacteria CORRECT ANS-

streak plate




In what orientation do you incubate the plates after they have been streaked? CORRECT

ANS-agar half bottom




What must you do to the loop before every use? CORRECT ANS-flame it




T/F: The last thing you do before leaving the lab is take off your lab coat CORRECT ANS-

false




streaked plate method CORRECT ANS-culture is spread out on agar to make isolated

colonies




solid media (agar plates) CORRECT ANS-used to isolate bacteria




liquid culture media (broth) CORRECT ANS-used to grow cells to high densities

, FINAL EXAM: MCB 2610 Lab final Exam with Certified
Solutions

T/F: When making a smear of the culture, you heat fix the slide before air drying it completely

CORRECT ANS-false




What is the magnification value of the ONLY objective that should come in contact with

immersion oil? CORRECT ANS-100x




Pertaining to the Gram stain, cells that retain the primary stain and appear purple are said to

be Gram CORRECT ANS-positive




What cellular structure does the counterstain safranin stain? CORRECT ANS-outer

membrane




direct stain CORRECT ANS-stains the organism itself




indirect or negative stain CORRECT ANS-stains the background

, FINAL EXAM: MCB 2610 Lab final Exam with Certified
Solutions
simple stain CORRECT ANS-use only one stain to determine cell morphology, size and

arrangement




Gram staining stains the cell __________, while spore staining stains for __________.

CORRECT ANS-cell envelope, endospores




Gram Positive cell characteristics CORRECT ANS-thick peptidoglycan layer, retains crystal

violet, no outer membrane




Gram Negative cell characteristics CORRECT ANS-thin layer of peptidoglycan, doesn't retain

crystal violet, has outer membrane, safranin counterstains membrane pink




Gram stain steps and time CORRECT ANS-1. crystal violet, 1 minute, 2. iodine, 1 minute, 3.

ethanol, 15-30 seconds, 4. safranin, 30 seconds




Why do bacteria form endospores? CORRECT ANS-under environmental stress




In a spore stain, endospores retain _________ (what color). CORRECT ANS-malachite green

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