Mrvp - Study guides, Class notes & Summaries

MIP 302 FinalPurpose of Blood Agar - correct answer See if bacteria has hemolysis abilities Purpose of Catalase Test - correct answer Determine if bacteria produces catalase Purpose of Indole Test - correct answer See if bacteria produces tryptophanase Medium for Indole test - correct answer Tryptone broth Purpose of Klinglers Iron Agar - correct answer See ability of bacteria to ferment glucose and/or lactose and the ability to produce CO2 and H2S gases Purpose of Lysine Decarbox...

Purpose of Blood Agar - correct answer See if bacteria has hemolysis abilities Purpose of Catalase Test - correct answer Determine if bacteria produces catalase Purpose of Indole Test - correct answer See if bacteria produces tryptophanase Medium for Indole test - correct answer Tryptone broth Purpose of Klinglers Iron Agar - correct answer See ability of bacteria to ferment glucose and/or lactose and the ability to produce CO2 and H2S gases Purpose of Lysine Decarboxylase Broth -...

Pre Lab Assignment 4 Phenol Red Broth, MRVP, Nitrate Reduction, Cat, Ox

Exercise on Phenol Red Broth Analyze the test tubes blow and answer the following three questions: 1 2 3 4 5 1. The PR sucrose tube (#2) was yellow in color after inoculating them with an organism and incubating for 24 hrs. What is your analysis/interpretation of these results (yellow color)?  My analysis/interpretation is that there was fermentation with acid end products; also, there was no gas produced. 2. The PR sucrose tube (#5) was pink in color after inoculating them with an o...

MIP 302 Final Test with Correct Answers What is Pseudosel selective for? - Answer-Pseudomonas Aerugionosa What is Pseudosel Differential for? - Answer-Isolation and identification of Pseudomonas aeruginosa What is in Pseudosel agar that makes it selective? - Answer-Cetrimide What pigments does P. Aeruginosa produce - Answer-Pyocynanin Is Pseudomonas Agar F selective or differential? - Answer-It is a differential medium, based on ability to produce fluorescein. What medium is the...

Carbohydrates composed of - ANSWER-monosaccharides Monosaccharides - ANSWER-glucose, fructose, galactose Polysaccharides - ANSWER-sucrose, lactose, maltose Disaccharides - ANSWER-starch in plants & glycogen in humans How can microorganisms be distinguished? - ANSWER-by whether they can break down monosaccharides, but not polysaccharides etc. -Not many organisms can break down starch 6 Tests for Carbohydrate Catabolism: - ANSWER-1. Oxidative Fermentation 2. Starch Hydrolysis 3. ...

What does IMViC stand for? - Answer-Indole, Methyl Red, Voges-Proskauer, and Citrate test. What is the IMViC test used for? - Answer-Used to identify if gut bacteria to ensure water doesn't have pathogens such as salmanella. Indole test - Answer-Detects the presence of tryptophanase to break down tryptophan into indole. Indole test indicator - Answer-Kovac's reagent Positive indole test result - Answer-Produces red top layer Negative indole test result - Answer-Produces colo...

Microbiology Lab Exam 1 Questions and Answers Rated A when viewing a smear with the oil immersion lens the final magnification is 1000x;10x ocular x 100X objective what is the purpose of heat fixing kill the bacteria, adhere to glass anddenature the proteins of bacteria name oneof the stains or regents used in the gram stain procedure gram crystal violet, gram iodine, gram sfaranin, 95% ethanol why are aseptic techniques used in the lab? to ensure that we do not contaminate what we are workin...

Simmons citrate test: enzyme correct answer: citrate permease Simmons citrate test: pH indicator correct answer: Brothymol blue Results of a positive Simmons citrate test correct answer: Turns blue can use sodium citrate as sole carbon source Results of a negative Simmons citrate test correct answer: Stays green color --> pH doesn't change doesn't contain the enzyme Malonate test: enzyme correct answer: Succinate dehydrogenase What does the Malonate test do? correct an...

Starch agar - ANSWER-¬Tests for the presence of amylase, an exoenzyme capable of breaking down starch ¬Media is supplemented with starch ¬Iodine reagent will react with the starch to turn the media black, any area of clearing around the bacterial growth would indicate the presence of amylase ¬Bacteria that are amylase + will show an area of clearing usually both with and without the iodine addition Spirit Blue Agar - ANSWER-¬Tests for the presence of lipase, an exoenzyme that breaks d...