Cgmbs - Study guides, Class notes & Summaries

CGMBS EXAM 1 (STUDY) QUESTIONS WITH SOLVED ANSWERS GRADED A+

CGMBS FINAL EXAM REVIEW QUESTIONS & ANSWERS RATED 100% CORRECT!!

CGMBS EXAM 5 (2024) QUESTIONS WITH PASSED SOLUTIONS!!

DNA polymerase delta - Answer-eukaryote: completes replication on the *lagging* strand; binds more tightly to DNA sliding clamp nucleotide and base excision repair Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping them from re-pairing topoisomerase 1 - Answer-Making single-stranded DNA breaks to relieve supercoiling at origin topoisomerase 2 - Answer-An enzyme that breaks both strands (a DNA double helix), rotates the ends, and seals the break. *USES ATP*...

Sequencing of the DNA region encoding 16S rRNA is performed to determine - ️️the evolutionary and genetic relatedness of microorganisms and has driven changes in microorganism nomenclature Homologous extrinsic control - ️️target-derived control with a non-target-derived sequence insert; control is added to every sample after nucleic acid extraction and before amplification Smallpox must be handled only in approved laboratories - ️️level 4 containment When processing whole bloo...

Warfarin (Coumadin) - ️️CYP2C9 and VKORC1 - Vitamin K antagonist; metabolized primarily via oxidation in the liver by CYP2C9 and exerts its anticoagulant effect by inhibiting the protein vitamin epoxide reductase complex, subunit 1 (VKORC1) Chronic Myelogenous Leukemia (CML) - ️️t(9;22) aka Philadelphia Chromosome, translocation occurs within two genes: the breakpoint cluster region (BCR) on chr.22 and the cellular counter part of the Abelson Leukemia virus tyrosine kinase (c-abl) o...

DNA was isolated from a patient and cut with HaeIII. The resulting fragments were separated by gel electrophoresis and transferred to a membrane. The fragments were allowed to hybridize to a probe, and the resulting band pattern was compared with that seen in another sample of DNA. Which of the following methods is described? a. RFLP by southern blot b. VNTR by PCR c. SNP by sequencing d. STR by PCR - ️️RFLP by southern blot Which of the following is used as a marker of gender? a. D...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...

ASCP CGMBS Exam Study Questions and Complete Solutions Graded A+