Protein - Study guides, Class notes & Summaries
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED 
E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY 
Lee Jia Yi 
 (12) 
Abstract 
In this study, a recombinant histidine-tagged 
green fluorescence protein(His6-GFP) was 
produced by E.coli XL1 cells followed by 
immobilized metal affinity chromatography 
(IMAC) using Nickel-agarose beads for 
purification. The GFP template was first 
amplified from pE-GFP vector using 
polymerase chain reaction (PCR). The primers 
c...
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Test Bank for Biochemistry, 7th Edition by Garrett Grisham
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Test Bank for Biochemistry 7e 7th Edition by Charles M. Grisham, Reginald H. Garrett. Full Chapters test bank are included - Chapter 1 to 32 
 
Part I: MOLECULAR COMPONENTS OF CELLS. 
1. The Facts of Life: Chemistry is the Logic of Biological Phenomena 1. 
2. Water: The Medium of Life. 
3. Thermodynamics of Biological Systems. 
4. Amino Acids and the Peptide Bond. 
5. Proteins: Their Primary Structure and Biological Functions. 
6. Proteins: Secondary, Tertiary and Quaternary Structure. 
7. Carbo...
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NUR 215 Exam 3: Modules 7-10 Latest Update Graded A+
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NUR 215 Exam 3: Modules 7-10 Latest 
 
Update Graded A+ 
 
Full Liquid Diet 
clear liquids plus liquid dairy products, all juices, pureed vegetables 
transition to soft diet, acute gastritis 
Mechanical Soft Diet 
clear and full liquids plus diced or ground foods 
Soft/Low-Residue Diet 
foods that are low in fiber and easy to digest (dairy products, eggs, ripe bananas) 
transition from FL to reg diet 
High Fiber Diet 
whole grains, raw and dried fruits 
Pureed Diet 
foods have been blenderized t...
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TEST BANK for Nester's Microbiology: A Human Perspective 10th Edition by Denise Anderson, Sarah Salm, Mira Beins and Eugene Nester. All Chapters 1-30 Updated A+
- Exam (elaborations) • 885 pages • 2023
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TEST BANK for Nester's Microbiology: A Human Perspective 10th Edition by Denise Anderson, Sarah Salm, Mira Beins and Eugene Nester. All Chapters 1-30 Updated A+All Chapters 1-30. (Complete Download) 1084 Pages. TABLE OF CON TENTS Chapter 1: Humans and the Microbial World Chapter 2: The Molecules of Life Chapter 3: Cells and Methods to Observe Them Chapter 4: Dynamics of Microbial Growth Chapter 5: Control of Microbial Growth Chapter 6: Microbial Metabolism: Fueling Cell Growth Chapter 7: The Bl...
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NSG222/ NSG 222 Exam 3: (Latest 2024/ 2025 Update) Family Nursing Review| Questions and Verified Answers| 100% Correct| Grade A – Herzing
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NSG222/ NSG 222 Exam 3: (Latest 2024/ 2025 Update) Family Nursing Review| Questions and Verified Answers| 100% Correct| Grade A – Herzing 
 
Q: placenta previa 
 a placenta that is implanted in the lower portion of the uterus, partially or completely blocking cervical opening 
 
 
Q: risk factors for placenta previa 
 
Answer: 
 - prior C-section 
- maternal age >35 
- smoking 
- diabetes 
- short interval between pregnancies 
 
 
Q: nursing management for placenta previa 
 
Answer: 
 - avo...
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Test Bank for Campbell Biology in Focus, 4th Edition by Lisa A. Urry | Latest Edition
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Test Bank for Campbell Biology in Focus 4e 4th Edition by Lisa A. Urry | Latest Edition with all chapters. Chapter 1 to 43 (complete) are included 
 
Introduction: Evolution and the Foundations of Biology 
UNIT 1: CHEMISTRY AND CELLS 
The Chemical Context of Life 
Carbon and the Molecular Diversity of Life 
A Tour of the Cell 
Membrane Transport and Cell Signaling 
An Introduction to Metabolism 
Cellular Respiration and Fermentation 
Photosynthesis 
The Cell Cycle 
UNIT 2: GENETICS 
Meiosis and...
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NURS 5315: Advanced Pathophysiology UTA Exam 1 LATEST UPDATED 2024
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NURS 5315: Advanced Pathophysiology UTA Exam 1 LATEST UPDATED 2024 
 
Atrophy Correct Answer: E. Cells decrease in size 
P. Still functional; imbalance between protein synthesis and degradation. Essentially there is an increase in the catabolism of intracellular organelles, reducing structural components of cell 
Physiologic: thymus gland in early childhood 
Pathological: disuse (muscle atrophy d/ decrease workload, pressure, use, blood supply, nutrition, hormonal stimulation, or nervous stimula...
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Exam 1: NUR242/ NUR 242 (Latest 2023/ 2024) Medical-Surgical Nursing Exam | Questions and Verified Answers| 100% Correct| Grade A- Galen
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Exam 1: NUR242/ NUR 242 (Latest 2023/ 2024) Medical-Surgical Nursing Exam | Questions and Verified Answers| 100% Correct| Grade A- Galen 
 
Q: A pt asks you why what he eats has anything to do with wound healing. What is your response? 
 
 
Answer: 
Successful healing of pressure injuries depends on adequate intake of calories protein, vitamins, minerals and water. 
 
 
 
Q: After receiving shift report, the night nurse looks at the lab values for a patient with cellulitis. What abnormal lab val...
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ATI pharm practice B 2023 with 100%Verified Answers.
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ATI pharm practice B 2023 with 100%Verified Answers. 
ATI pharm practice B 
1. A nurse is caring for a client who is receiving haloperidol. The nurse should 
identify which of the following findings as an adverse effect of the 
medication? 
A. Akathisia 
B. Paresthesia 
C. Excess tear productionD. Anxiety: A. Akathisia 
2. A nurse is providing teaching to a client who is to start taking 
sumatriptan. Which of the following adverse effects should the nurse 
instruct the client to monitor for and ...
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Final Exam: NR547/ NR 547 Differential Diagnosis in Psychiatric-Mental Health Across the Lifespan Practicum Exam Review| Weeks 5-8 Covered| Complete Guide with Questions and Verified Answers (New Update 2023/ 2024) - Chamberlain
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Final Exam: NR547/ NR 547 Differential Diagnosis in 
Psychiatric-Mental Health Across the Lifespan Practicum 
Exam Review| Weeks 5-8 Covered| Complete Guide with 
Questions and Verified Answers (New Update 2023/ 2024) - 
Chamberlain 
QUESTION 
tell the difference between delirium and dementia 
 
 
Answer: 
onset 
attention 
do symptoms fluctuate? 
 
 
 
QUESTION 
Alzheimer's disease 
 
 
Answer: 
-type of dementia 
• 60-80% of dementias 
-neurodegenerative disease 
-Hallmarks: 
• pla...
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