Denaturation - Study guides, Class notes & Summaries

PCR allows for: - amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? - well designed primers Name 3 variables that influence the success of a PCR reaction - 1. quality of template DNA 2. cycling conditions 3. specificity and stability of PCR primers The target sequence determines what criteria in primer design for PCR? - 1. primer length 2. % GC content 3. m...

Choose the correct coding strand (non-template) from this template (non-coding) DNA: 5' ATC GAT 3' 3' TAG CTA 5' If one strand of chromosome 2 has a DNA sequence that consists of this: 5' AGA CTA ATT GCC 3', what will be the composition of the complementary DNA strand? (Select all that apply) 3' TCT GAT TAA CGG 5' 5' GGC AAT TAG TCT 3' The following sequence is the coding strand of the collagen gene: 5' GGC AAA TAT CGA 3' What is the sequence of the corresponding mRN...

Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR - 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: re...

Which of the four elements in proteins differentiated this class of substances from carbohydrates and lipids? - Nitrogen Which of the proteins is soluble in water? - Albumin How are amino acids joined together to form a chainlike structure? - Peptide bonding Proteins may become denatured when subjected to mechanical agitation, heat, or chemical treatment. What does denaturation of proteins refer to? - Alteration of tertiary structure Which aminoacidopathy is NOT caused by an enzyme deficie...

MT AAB Chemistry Questions with 100% Correct Answers | Verified | Latest Update 2024 Which of the four elements in proteins differentiated this class of substances from carbohydrates and lipids? - Nitrogen Which of the proteins is soluble in water? - Albumin How are amino acids joined together to form a chainlike structure? - Peptide bonding Proteins may become denatured when subjected to mechanical agitation, heat, or chemical treatment. What does denaturation of proteins refer to? - Altera...

Biochemistry Study Guide Questions and Answers Rated A+ What unique feature of enzymes allows them to be highly specific for their substrates? The unique three-dimensional shape of an enzyme allows it to fit only specific substrates, much like a key in a lock. How does the structure of glycogen contribute to its function as an energy reserve? Glycogen's branched structure allows for rapid mobilization of glucose when energy is needed quickly. What is the significance of the ...

Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR - 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: re...

BIO 101 Exam 2 WVU Salihu with Complete Solutions pH scale Acidic: 0-6.9 Neutral: 7 Basic (alkaline): 7.1-14 acidity amount of hydrogen ions in a solution (called pH) acids vs. bases ions acids- more H+ bases- more OH- macromolecules sequence of simple molecules; all composed of C, H, and O types of macromolecules carbohydrates, proteins, lipids, and nucleic acid elements in DNA contains the same elements as other macromolecules, except it has N and P monomers of compl...

WGU 785 Final Exam Questions & Answers 2024/2025 Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - ANSWERSHis daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - ANSWERSAutosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - ANSWERSThe process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps ...

BCH 361 EXAM 2 QUESTIONS WITH ALL VERIFIED ANSWERS What is the purpose of the oxyanion hole in chymotrypsin? - Answer-The oxyanion hole is a structure at the active site of chymotrypsin that stabilizes the tetrahedral intermediate in the proteolysis reaction and facilitates the formation of the acyl-enzyme intermediate. What caused a "burst" of activity followed by a steady-state reaction when chymotrypsin was studied in the milliseconds subsequent to mixing the enzyme and substrate? - An...