Bcmb - Study guides, Class notes & Summaries

How is a tertiary structure held together? - correct answer the hydrophobic effect, Hydrogen bonds, and ion-ion interactions What would be the consequence of changing an amino acid that helps to hold together tertiary structure? - correct answer Changing the amino acid would change the structure therefore making it non-functional. How is a quarternary structure held together? - correct answer By the hydrophobic effect, hydrogen bonding, ion-ion interactions and Van der Waals interact...

The activity of an enzyme can be regulated by a: competitive inhibitor binding to the active site. competitive inhibitor binding to the ES complex. noncompetitive inhibitor binding to the ES complex. All of the above Only the first and third answers are correct. - Only the first and third answers are correct. In chymotrypsin, the catalytic triad is made up of which three amino acids? Asp, His, Cys Asp, His, Ser Asp, Asp, Ser Asn, His, Ser Asn, His, Cys - ...

______ methodology allows for the study of complex *cellular regulation of genes *the role of specific amino acid residues in protein structure, catalytic function and ligand binding capacity *mechanisms involved in infectious and genetic disease - Answer Site directed mutagenesis

The success of a PCR reaction is mostly dependent on what? - well designed primers Primer design can also be applied to what? - - probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - - Primer length - Percent GC content - Melting temperature - Sequence How are Primer...

Briefly describe Bartter's syndrome and the role of the gene KCNJ1. Answer> Bartter's syndrome is a genetically inherited disease that causes excess salt loss and the hardening of kidney tissue. Type II Bartter's syndrome, which is antenatal, is caused by a mutation in the KCNJ1 gene. Mutations in KCNJ1 gene change/delete amino acids in ROMK which results in a dysfunctional/nonfunctional protein. ROMK moves potassium across kidney cell membranes so dysfunctional ROMK causes pota...

Briefly describe Bartter's syndrome and the role of the gene KCNJ1. Bartter's syndrome is a genetically inherited disease that causes excess salt loss and the hardening of kidney tissue. Type II Bartter's syndrome, which is antenatal, is caused by a mutation in the KCNJ1 gene. Mutations in KCNJ1 gene change/delete amino acids in ROMK which results in a dysfunctional/nonfunctional protein. ROMK moves potassium across kidney cell membranes so dysfunctional ROMK causes potassium deficiency in ...

Catabolic pathway correct answer: break down fuel molecules to make ATP Anabolic pathway correct answer: use ATP and reducing power to make complex molecules Would a high [products]/[substrates] ratio contribute favorably or unfavorably to the overall ΔG of a reaction? correct answer: unfavorably Would a reaction with a negative ΔG°ʹ have a higher concentration of products or a higher concentration of substrates at equilibrium? correct answer: more products than substrates at eq...

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

summarize the overall goal of the pyruvate dehydrogenase complex (PDH) - correct answer an enzyme that catalyzes the transfer of pyruvate to acetyl CoA does low energy charge inhibit or activate the PDH complex? - correct answer activate does phosphorylation activate or inhibit the PDH complex? - correct answer inhibit do high levels of acetyl coA activate or inhibit the PDH complex? - correct answer inhibit at E2 do high levels of NADH activate or inhibit the PDH complex? - ...

Glycoside hydrolases - Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Vector NTI Advance - - Allows you to plan your experiment and ensure that it results in the desired product Benefits of in silico - - Predicts restriction fragment s...