Polymerase chain - Study guides, Class notes & Summaries

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

Prescott's Microbiology, 11e (Willey) Chapter 1 The Evolution of Microorganisms and Microbiology 1) Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. Answer: FALSE Topic: Taxonomy of Microorganisms Bloom's/Accessibility: 2. Understand / Keyboard Navigation ASM Topic: Module 01 Evolution ASM Objective: 01.05 The evolutionary relatedness of organisms is best reflected in phylogenetic trees. Learning Outcome: 01.02a Propose a timeline of the ori...

We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. a) DNA has to be isolated from the cells What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b) Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip d) Put ...

WGU Biochemistry OA Review Questions and Answers WITH VERIFIED SOLUTIONS GRADED A+ The polymerase chain reaction is a tool used to study protein structure. True False

What needs to be done to the sample of blood before PCR? DNA must be isolated from the cells. How did you collect quids in the lab? A pipette What do you need to do each time before using a pipette to collect liquid? Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? to avoid cross contamination For wh...

What is the function of DNA? DNA stores information Which Cell contains DNA? A white blood cell DNA is located instead the... Nucleus What type of structure does DNA have? double helix What is the complementary DNA sequence for the following sequence: 5'-AATTCGCGG-3' 3'-TTAAGCGCC-5' Which of the following nucleotides is not a part of DNA? Uracil Where is uracil used? In RNA What approach would you take to find out which blood sample cam...

***Download Test Bank Immediately After the Purchase. Just in case you have trouble downloading, kindly message me, and I will send it to you via Google Doc or email. Thank you*** Biology: The Core 3rd Edition Exam Questions and 100% Correct Answers| Eric J. Simon The Test Bank for Biology: The Core, 3rd Edition by Eric J. Simon is a comprehensive study tool designed to aid in the understanding of fundamental concepts in biology. It contains a series of questions and answers that align with th...

RNAS C Practice Test Review 437 Questions with Verified Answers A capacitor and resisitor in parallel - CORRECT ANSWER will have same voltage across since in parallel. if in series, voltage drops would occur they can be treated together If the absolute pressure of a gas is increased from 3 atm to 4 atm at constant volume, then the absolute temperature of the gas will increase by: A. 25%. B. 33%. C. 67%. D. 75% - CORRECT ANSWER B. Assuming the validity of the Ideal-Gas law, PV = n...

Major Steps of the Central Dogma correct answers Replication, transcription, and translation. DNA correct answers What is the main product of replication? RNA correct answers What is the main product of transcription? Proteins correct answers What is the main product of translation? Promoter correct answers A region of DNA that provides a secure binding site for RNA polymerase and transcription factors. mRNA, rRNA, tRNA correct answers Three types of RNA generated by transcr...

WGU Biochemistry OA (Latest 2022/2023) Verified Answers Bohr Effect chart (low pH) C- High CO2 H- High H+ A- Acidic R-Release of O2(rt. Shift) T- tense state (in tissues) Primary structure Sequence of amino acids that make up a protein Secondary Structure Sequence of amino acids fold. Tertiary Structure -more folding in a 3D shape. -R groups (side chains) Quaternary Structure Protein has more than 1 polypeptide chain. Chemical reaction -absorb and release en...