Mbascp - Study guides, Class notes & Summaries

MB ASCP Study Aids Buckingham Ch. 7 2023 Updated.Polymerase Chain Reaction (PCR) - ANSWERPrimer-directed DNA polymerization in vitro resulting in amplification of specific DNA sequences. Target amplification - ANSWERTarget: Specific sequences or regions that are detected in an analytical procedure. Amplification: Replication/copying Target amplification: replication of a specific target region of DNA. Template - ANSWERSingle stranded nucleic acid used as a guide for replication or synthesi...

Molecular Diagnostics Chapter 4(Well Solved). gel electrophoresis -ANSWERS. Procedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel Is the anode positive or negative? What about the cathode? -ANSWERS. Positive, negative DNA/RNA are positively/negatively charged? They migrate towards anode or cathode? -ANSWERS. negatively, anode Do large or small size DNA fragments migrate faster?...

MB(ASCP) Genes, Drugs, And Translocations Verified Questions And Answers 2024 MB(ASCP) Genes, Drugs, And Translocations Verified Questions And Answers 2024

Translocations Advanced 2023.t(1,13) - ANSWERPAX3/7 + FKHR (there are two of these) Rhabdomyosarcoma t(2:13) - ANSWERPAX3/7 + FKHR (there are two of these) Rhabdomyosarcoma t(11:22) - ANSWEREwing sarcoma, childhood neoplasms, EWSR1 t(x:18) - ANSWERsynovial sarcoma, SS18, reciprocal translocation ss18/syt—>ssx1/ssx2 t(9:22) - ANSWERPhiladelphia chromosome, CML (BCR-ABL activation, tyrosine kinase oncogene) t(15:17) - ANSWERacute promyelocytic leukemia PML/RARA t(8:14) ...

Chapter 1 MB Buckingham ASCP New Version. Johann Friedrich Miescher - ANSWERDiscovered DNA 1869. Extracted a viscous substance from isolated white blood cells. Prepared nuclei of cells by adding pepsin. Chemical analysis of this substance found in nuclei ("nuclein") showed that it was 14% N and 2.5% P, different from other known biochemicals. Nucleotides - ANSWERBuilding blocks of DNA, 700 kilodaltons each. Consists of a 5 C sugar where the 1st C is covalently bonded to a N base,...

ASCP Technologist in Molecular Biology board exam prep Technologist in Molecular Biology, MB(ASCP) board exam prep • Pyrimidine • One carbon ring • Cytosine, Thymine, Uracil Purine • Two carbon rings • Adenine, Guanine How are nucleotides joined together? • Condensation to form phosphodiester bond What is the function of mRNA? • Carries genetic info out of nucleus • Transcript translated to protein

1. Which PCR method has the highest specificity? - ANS>>Nested PCR 2. After an amplification procedure followed by gel electrophoresis, you took a photo of your gel. You notice consistent bands in all your gel lanes. However, you also see what appears to be a faint but noticeable band in your reagent blank lane. What is your best explanation for the reagent blank band? - ANS>>There was obvious contamination in this PCR reaction 3. A technologist uses the spectrophotometer to qu...

Technologist in Molecular Biology, MB(ASCP) board exam prep 2024 with 100% Correct Answers

Certification Exam Cards(complete solution graded).When handling blood spots, one should A. be even more careful when handling blood than other body fluids. B. protect from cross contamination from other blood spots C. store at room temperature up to 24 hours D. store at 4 degrees C for up to 7 years - ANSWERB. protect from cross contamination from other blood spots In handling blood serum, one should A. store in eppendorf tube in refrigerator indefinitely B. separate from ce...