Isoelectric - Study guides, Class notes & Summaries

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024 COMPLETE SOLUTIONS LATEST 2023/2024 Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Use d in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solu...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

ACS Biochemistry Final Exam Questions and Correct Solutions 2024 pKa (chapter 3) - Answer -measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - Answer -compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - Answer -dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - Answer -the ch...

The Structure of Amino Acids correct answers Amino group, carboxyl group, and R group attached to alpha carbon What is the carbon attached to the amino group, carboxyl group, and R group? correct answers Alpha carbon What are the carbons after the alpha carbon? (in the correct order away from the alpha) correct answers Beta, gamma, delta What is the pI? correct answers Isoelectric point of the Amino Acid titration curve What is the point of the Amino Acid curve? correct answers To ta...

Amino Acid and Side Groups correct answers - Negative Side Groups = Acidic - Positive Side Groups = Basic Absolute Configuration correct answers refers the R/S naming convention. Some amino acids are R, and some are S. Relative Configuration correct answers - D/L naming convention. - All biologically produced amino acids are in the L configuration. Conformational stability correct answers - Temperature: Denatures second, third, and fourth tertiary - pH: Third and fourth; Destroys io...

ASCP MLT Chemistry Exam Questions with Answers Graded A+ What is the principle of flame photometry? - Answer-emission of color when an element is burned What is the principle of atomic absorption spectrophotometry? - Answer-It measures energy emitted by ultraviolet rays What is the light source in atomic absorption instrumentation? - Answer-cathode lamp Quenching is a disadvantage in what type of instrumentation? - Answer-fluorometry What analytical method measures the amount of lig...

electrically polarized at rest - inside of heart cell has a high potassium concentration, whereas the outside has a higher concentration of NA -higher positive charge outside the cell cause more negative charge inside cell electrical potential difference between the charges outside and inside of the cell wall Brainpower Read More 0:09 / 0:15 Depolarization NA moves into the cell rapidly in response to an electrical impulse - inside cell loses negative potential Replola...

Electrophysiology - ACCURATE ANSWERS 1. Automaticity - ability to create electrical impulse spontaneously - every cell contains 2. Excitability - ability to respond to outside stimulus - ready to transmit/contract 3. Conductivity - receive impulse, conduct to nearby cells 4. Contractility - ability to shorten, or contract Polarization - ACCURATE ANSWERS alive and ready for signal - inta/extra cellular ions aligned Depolarization - ACCURATE ANSWERS ions shift, creating wave as it ...

(*Molecular Biology) Hydrolases correct answers Enzymes that catalyze reactions between a specific substrate and water in order to break a covalent bond. (*Molecular Biology) pI (Isoelectric point) of an amino acid correct answers The pH at which an amino acid carries no electrical charge (average of the pKa's of the carboxyl and amino group) (*Genetics) Silent Mutations correct answers Mutations that do not change the amino acid sequence (*Molecular Biology) When are Amino aci...

5 lead electrode placement - go in intercostal space! *Smoke over fire, snow over grass, and brown in middle!* 6 steps of rhythm strip analysis - 1. determine if regular or irregular 2. calculate rate 3. evaluate the p & QRS waves 4. measure the PR interval 5. measure the QRS 6. interpret rhythm A-Fib etiology - - Advanced age - MI - CAD - Valvular disease - Congenital heart defects - Hyperthyroidism - Stimulant medication abuse A-Fib s/sx - u-"F" fibrillatory waves -Ø discern...