Cas9 - Study guides, Class notes & Summaries

The two polynucleotide chains in DNA are held together primarily by H bonds between A and U as well as between G and T? a. True b. False correct answers B (DNA does not have Uracil, it has Cytosine) If there is 12% of A in a DNA molecule, then the % of G must be? a. 12% b. 30% c. 368 d. 76% e. none of these are correct correct answers E (A=T and C=G, A+G/T+C=1) The catalytic molecule responsible for making a peptide bond during translation (protein synthesis) in cells is? ...

CMMB 403 final exam what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - Answer ️️ -- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - Answer ️️ -1. non-homologous end joining (NHEJ) - nat...

How does CRISPR work naturally? Correct Answer Clustered Regularly Interspaced Short Palindromic Repeats - bacterial defense mechanism - bacteria have short palindromic repeats separated by spacer regions which are segments of DNA from past viral exposure - when attacked again by same virus, the spacer region is used as a template and the guide RNA (tracrRNA) helps recognize the viral DNA and degrade it using Cas9 nuclease at the specific site - need PAM (helps distinguish between self and n...

Module 6: Genetic Engineering Exam Questions With Verified Answers. How are ds breaks repaired - answernon-homologous end joining (NHEJ) homologous directed recombination (HDR) non-homologous end joining (NHEJ) definition - answerligated 2 broken ends together (not template repaired) typically results in insertions or deletion homologous directed recombination (HDR) definition - answertemplate dependent pathway for DSB repair can insert donor molecule at target types of application of ...

CRISPR Cas-9 Questions UPDATED Exam Questions and CORRECT Answers How many nucleotides long is the guiding region of the sgRNA? - Correct ANSWER- 20 nucleotides Does the sgRNA bind to the PAM? - Correct ANSWER- Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - Correct ANSWERIt cuts the DNA 3 nucleotides upstream of the PAM

CRISPR Cas-9 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Describe i...

Causes of chromosomal aberrations - errors in chromosome segregation (causes whole chromosome aneuploidy) and/or DNA damage repair (causes structural variation) Anneal - the ability of two complementary nucleic acids to align in an opposing orientation to allow the nucleotide bases of one strand to form hydrogen bonds with the nucleotide bases of the complementary strand; used in microarrays to bind DNA and show fluorescence Absolute risk - A person's chances of developing a disease or dis...

CMMB 403 final Exam New Year 2024| Questions and Answers| Certified Quality Graded A+ what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 1. non-homologous end jo...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...