Phoresis - Study guides, Class notes & Summaries

Gel Electrophoresis Questions and Answers Already Passed What property of DNA allows it to migrate towards the positive electrode during gel electrophoresis? DNA is negatively charged due to its phosphate backbone, causing it to move towards the positive electrode when an electric field is applied. In gel electrophoresis, what role does the gel matrix play in the separation of molecules? The gel matrix acts as a sieve, allowing smaller molecules to pass through more easily while re...

Gel Electrophoresis Questions and Answers Already Passed What type of gel is typically used for separating nucleic acids? Agarose gel is typically used for separating nucleic acids like DNA and RNA. How does the size of DNA fragments influence their migration rate in gel electrophoresis? Smaller DNA fragments migrate faster through the gel than larger fragments due to less resistance in the gel matrix. What is the purpose of applying a dye to the gel before electrophoresis? Dyes ...

Gel Electrophoresis (Honors Bio) Questions and Answers 100% Pass How do scientists visualize DNA after it has been separated by gel electrophoresis? Scientists use staining agents, such as ethidium bromide or SYBR Green, which bind to DNA and make it visible under UV light. What would happen if the gel concentration is too high in gel electrophoresis? If the gel concentration is too high, the pores would be too small, and larger DNA fragments would have difficulty moving through the g...

Gel Electrophoresis LAB 4 Latest Update Graded A+ How do we prepare our samples before they hit the gel? Samples are mixed with loading dye to add color and density, ensuring they sink into the wells and are visible during the run. What magical ingredient makes the gel strong enough to hold DNA? Agarose is the magical ingredient that forms the gel, providing a matrix that helps separate DNA based on size. How does the DNA fragment size affect its journey through the gel? Smaller...

Electrophoresis Steps Latest Update Graded A+ What is the first step in preparing for gel electrophoresis? The first step is to prepare the agarose gel by dissolving agarose powder in a buffer solution and heating it until it melts. How do you make sure the gel has wells for loading samples? You insert a comb into the gel mold while the agarose is still liquid; once it cools, the comb creates wells for the samples. Why is it important to let the gel solidify before running the ex...

Biology- Gel Electrophoresis Questions and Answers Graded A+ How does the electric field strength affect the resolution of a gel? A stronger electric field increases the speed of migration but can reduce resolution; a balanced field strength is crucial for optimal separation. What precautions should be taken when using UV light to visualize DNA bands? Protective eyewear should be worn, and the exposure time should be minimized to avoid damaging the DNA and harming the eyes. What ...

Gel Electrophoresis Questions and Answers Graded A+ What is the relationship between gel concentration and the size of molecules being separated? Higher gel concentrations create smaller pores, which are more effective for separating smaller molecules, while lower concentrations allow for the separation of larger molecules. Describe how gel electrophoresis can be used to determine the purity of a DNA sample. By running a gel electrophoresis, one can observe the banding pattern of a D...

Gel Electrophoresis Virtual Lab Questions and Answers Rated A+ What is the primary purpose of using a virtual lab for gel electrophoresis? A virtual lab allows students to simulate the gel electrophoresis process, helping them understand the technique without the need for physical materials and minimizing safety risks. How can you determine the size of DNA fragments after running gel electrophoresis in a virtual lab? By comparing the bands of the sample against a DNA ladder or marke...