Pellissippi State Community College
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PEA 
1. When examining a PEA plate that has been inoculated with organism and 
incubated for 24 hrs, we noticed that the organism is growing well. What does 
this tell us about the organism’s cell wall? 
 The organism’s cell wall was not inhibited by the phenylethyl alcohol. 
Also, the organism most likely has a thick peptidoglycan layer that causes 
the cell wall to stay intact; having a thick peptidoglycan layer would 
make the organism a Gram positive. 
2. What is added to a PEA plate ...
Assignment 1 
Diseases of the Digestive System
Thioglycollate Exercise 
1. What is Thioglycollate broth used for? Be specific!!! 
A. create oxygen gradient C. prevent growth 
B. keep temp constant D. grow colonies 
Aerotolance Exercise 
2. Match the Following: 
b. anaerobe A. uses oxygen 
a. aerobe B. does NOT use oxygen 
Temperature Exercise 
3. I was really worried when I found out that the organism I had just eaten was a ___ 
a. psychrophile c. xanthophile 
b. mesophile d. thermophile 
Exercise 6-2 – Urine Culture 
4. Urine shoul
1. The Biol 2130 students and employees of a public school system developed 
diarrheal illness on April 2. The cafeteria served chicken that day. On April 1, part of 
the chicken was placed in water-filled pans and cooked in an oven for 2 hrs at a 
temp of 177oC. The oven was turned off and the chicken was left overnight in the 
warm oven. Symptoms of the disease included nausea, vomiting, cramping and a 
fever. All the patients recovered within 24 hrs. Two “serotypes” of a Gram positive, 
o...
BIOL 2130 Assignment 2-Diseases of the Respiratory System study guide spring 2023
PEA 
1. When examining a PEA plate that has been inoculated with organism and 
incubated for 24 hrs, we noticed that the organism is growing well. What does 
this tell us about the organism’s cell wall? 
 The organism’s cell wall was not inhibited by the phenylethyl alcohol. 
Also, the organism most likely has a thick peptidoglycan layer that causes 
the cell wall to stay intact; having a thick peptidoglycan layer would 
make the organism a Gram positive. 
2. What is added to a PEA plate ...
Exercise on Phenol Red Broth 
Analyze the test tubes blow and answer the following three questions: 
 1 2 3 4 5 
1. The PR sucrose tube (#2) was yellow in color after inoculating them with an organism 
and incubating for 24 hrs. What is your analysis/interpretation of these results (yellow 
color)? 
 My analysis/interpretation is that there was fermentation with acid end 
products; also, there was no gas produced. 
2. The PR sucrose tube (#5) was pink in color after inoculating them with an o...
Pre Lab Assignment 5 
Lysine Decarboxylation, Phenylalanine Deamination, SIM, & Citrate Tests 
Name: 
Instructor: 
Date: 
Lab Section: 
INSTRUCTIONS: 
 You can consult your lab manual pages, lab preparation sheets, and textbook for this 
exercise to answer the following questions. 
 Type your answers and print the page. 
 Bring the document to lab. 
Lysine Decarboxylation Exercise 
1. What are you removing when you decarboxylate a molecule? 
 When you decarboxylate a molecule, you ar...
Pre Lab Assignment 7 
 Mixed Unknown - 1
1. The primary indication for transferring a1. The primary indication for transferring a patient to a higher level trauma center is: patient to a higher level trauma center is:unavailability of a surgeon or operating roomunavailability of a surgeon or operating .multiple system injuries, including severemultiple system injuries, including severehead injury.resource limitations as determined by theresource limitations as determined by thetransferring ferring doctor.resourc...