Gfp - Guides d'étude, Notes de cours & Résumés

Portage Microbiology Exam #3 Questions With 100% Correct Answers What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer) - answer60 x 10 = 600x magnification True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy - answerFalse. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? (...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer) - Answer 60 x 10 = 600x magnification True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy - Answer False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast Which of the following could be seen clearly by the unaided eye? (multiple choice) - Answer Protozoa with diameter of 150um ...

LAB 9 - Green Fluorescent Protein (GFP) PurificationWhat is a protein? Proteins are long chains of amino acids, some of which are hydrophobic Explain the relationship between genes and proteins: Genes contain the genetic code which determine the amino acid composition of a protein. There is a unique gene for each protein within all of the cells of the body Using your own words, describe cloning: The duplication and propagation of a cell or organism. Describe how the bacte...

Which steps involved in the contraction of a skeletal muscle require binding and/or hydrolysis of ATP? I Dissociation of myosin head from actin filament II Attachment of myosin head to actin filament III Conformational change that moves actin and myosin filaments relative to one another IV Binding of troponin to actin filament V Release of calcium from the sarcoplasmic reticulum VI Reuptake of calcium into the sarcoplasm A. I, II, and III only B. II, III, and IV only C. I, III, and ...

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

Counseling and Feedback TLOs (Just a guide for what to takeaway from this section) 1. Review the four stages of counseling in the correct sequence. 2. Analyze at least ten forms of resistance. 3. Explain how counseling relates to leader development in at least three ways First Stage of Counseling Identify the Need for Counseling (require evaluation or command-directed counseling, counsel whenever focused, two-way communication aimed at the subordinate's development is needed) ...

IDENTIFICATION OF RECOMBINANT GFP PROTEIN USING CLONING, EXPRESSION AND PURIFICATION Abstract. 144 Green fluorescent protein is an useful tool to observe the presence of protein-protein interaction as well as gene expression(Chalfie et al., 1994). Which those experiment include amplification and recombination of GFP open reading from using RT-PCR which can clone GFP and insert into pQE30 vector with IPTG(Riedy et al., 1995). which is used to promote expression of N terminal hexahis...

PURIFICATION OF GFP FUSION PROTEINS WITH IMAC IN E. Coli CELLS ABSTRACT This report was performed to demonstrate techniques of purifying Green Fluorescent Protein (GFP). The main technique used for demonstration is by Immobilized Metal Affinity Chromatogrpahy (IMAC). The open reading frame for enhanced green fluorescent protein was subcloned into the pQE30 expression. The subsequent production of protein tagged N-terminally with hexahistidine, facilitated the purification by IMAC