Polymerase Guides d'étude, Notes de cours & Résumés

What needs to be done before PCH can be performed - Answer DNA has to be isolated from the Cells How did you collect liquid in a lab - Answer Using a pipette What do you need to do each time before using a pipette to collect liquid - Answer Put on a new sterile pipette tip What is the fiction of primers in a PCR reaction? - Answer Bind to specific sites on the DNA Wh is it important to change the pipette tip? - Answer To avoid cross contamination For which enzyme ar...

DNA Polymerase Study Guide Test. DNA Polymerase - CORRECT ANSWER Enzyme that assembles nucleotides into a new DNA strand DNA Polymerase I - CORRECT ANSWER DNA repair and some replication. Highest concentration DNA Polymerase II - CORRECT ANSWER DNA repair. Middle concentration DNA Polymerase - CORRECT ANSWER Have 3'-5' exonuclease; proofreading ability - keeps mutation rate below a certain level. Can only polymerize in a 5'-3' direction. Can only add nucleotides to an existing ...

We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. - Answer a) DNA has to be isolated from the cells What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b) Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip -...

What needs to happen before the blood sample can be used for PCR? - Answer DNA has to be isolated from the cells What is the function of primers in a PCR reaction? - Answer Bind specific sites on the DNA For which enzymes are nucleotides the substrate? - Answer DNA polymerase What is the template of the PCR? - Answer DNA What does DNA polymerase do? - Answer synthesizes new DNA strands Why is it important to change the pipette tip? - Answer To avoid cross contami...

What needs to be done to the sample of blood before PCR? - Answer DNA must be isolated from the cells. How did you collect quids in the lab? - Answer A pipette What do you need to do each time before using a pipette to collect liquid? - Answer Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? - Answer Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? - Answer to ...

Which strand is produced more rapidly? - ANSWER-Leading strand During DNA replication in the lagging strand there is production of this component? - ANSWER-Okazaki fragment How many "stop" codons exist? - ANSWER-3 The double helix of DNA is wrapped about 1¾ times around which of these components? - ANSWER-Nucleosome Which enzyme unwraps the double helix? - ANSWER-Helicase In the ribosome, what is the name of the bond that links one amino acid to the next? - ANSWER-peptide bonds...

This DNA amplification method amplifies the nucleic acid used as a probe? correct answer: Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? correct answer: DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? correct answer: Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? correct answer: Better specificity How does Ligase Chain Re...

AC-HPAT Prep (Biology, Chemistry, Physics, Math) 188 Questions With Complete Solutions. Most of the cell membrane's specific functions are controlled by: Proteins Alcoholic Fermentation is a form of: Anaerobic Respiration Phagocytosis is a form of: Endocytosis What is a pyruvate? Pyruvate is an important chemical compound in biochemistry. It is the output of the metabolism of glucose known as glycolysis. One molecule of glucose breaks down into two molecules of pyruvate, which are the...

Chronic Myeloid Leukemia (CML) t(9;22) BCR-ABL translocation Ewing sarcoma t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) t(9;22) BCR-ABL p190 protein Follicular Lymphoma t(14;18) BCL2 translocation Mantle Cell Lymphoma t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma t(8;14) MYC gene translocationRhabdomyosarcoma (alveolar); Cancer/solid tumor t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocation AML (Acute Myeloid Leukemia) t(8;21) inv(16) t(15;17) RUNX...