Totect - Study guides, Class notes & Summaries

What are the steps tissue must go through before it is viewed under a microscope? CORRECT ANSWER-1. Fixation 2. Processing 3. Embedding 4. Microtomy 5. Staining 6. Coverslipping Define fixation. CORRECT ANSWER-The preservation of cells and tissue in as life-like a way as possible by stabilizing the protein so that it is resistant to further changes. What are the functions of fixation? CORRECT ANSWER-1. To prevent putrefaction and autolysis 2. To help maintain proper relationships b/w...

Microscope - correct answers Used to for the magnification of the tissues and cells. Microtome - correct answers Used for the cutting of the wax and tissues. Block holder (chuck) - correct answers Holds the tissue in position. Knife and Knife carrier - correct answers For actual cutting of tissue sections. Pawl, Ratcher Feed Wheel & Adjustment screw - correct answers Lines up the tissue block in proper position with knife, adjusting thickness of tissues for successive sections. So...

For adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 CORRECT ANSWER-D. 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope CORRECT ANSWER-B. Polarizing microscope For most routine H & E staining, the sections should be 1 cel...

Histotechnology - NSH- Gross Dissection and Description at the Basic Level questions with correct answers

Histotechnology HTL Exam - Comprehensive Review questions with correct answers

What method is used to stabilize proteins making it resistant to further damage? CORRECT ANSWER-Fixation Name two methods of fixation? CORRECT ANSWER-Chemical reaction (formaldehyde) and physical (heat or dessication) What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** CORRECT ANSWER-bacteria or mold What factor causes autolysis if tissue is not fixed? **** CORRECT ANSWER-enzyme attack What are four functions of fixatives? CORRECT ANSWER-1. kills tissue ...

What are the steps of processing? CORRECT ANSWER-Fixation, (decalcification), dehydration, clearing, infiltration, embedding The step of processing removes water for the next subsequent step to occur (xylene and paraffin) CORRECT ANSWER-dehydration What accounts for the majority of tissue processing problems? **** CORRECT ANSWER-incomplete dehydration How does one solve incomplete dehydration? CORRECT ANSWER-Reprocess in water and then absolute alcohol. Or melt paraffin block, pat tissu...

Histotechnology Mid TermWhen is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, brit...

Acetic acid CORRECT ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde CORRECT ANSWER-Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal CORRECT ANSWER-This fixative is the smallest ...

Histotechnology - FixationThe primary purpose of fixation is to: a. stop enzymatic action b. remove the water from the tissue c. provide support for the tissue d. autolyze the tissue - correct answers a Which of the following fixatives contains picric acid, formalin, and acetic acid? a. Zenker b. Helly c. Bouin d. Zamboni - correct answers c The volume of fixative should exceed the volume of the tissue by: a. 1 time to 2 times b. 5 times to 10 times c. 15 times to 20 ...