Totect - Study guides, Class notes & Summaries

Histotechnology test 2What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 degr...

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

fixitive used for specimens - correct answers 10% neutral buffered Formalin Marking Margins - correct answers - to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station - correct answers phenolic disenfectant or gluteroldehyde how long is specimens stored for - correct answers 1-3 months some legal cases kept indefinitly Why is fresh tissue not stored? - correct answ...

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...

Periodic Acid Schiff stain demonstrates CORRECT ANSWER-Polysaccharides, neutral mucosubstances, and basement membranes Periodic acid and Chromic acid oxidise carbohydrates to CORRECT ANSWER-aldehydes What acid causes loss of quinoid structure in basic fuchsin? CORRECT ANSWER-Sulfurous acid The control of PAS is CORRECT ANSWER-Kidney The control for PAS-D is CORRECT ANSWER-Liver - one section "with" and one section "without" Alcian blue pH 2.5 demonstrates CORRECT ANSWER-Acid m...

affinity or avidity:a dye is attracted to a tissue CORRECT ANSWER-affinity affinity or avidity: how strongly a dye is attracted to a tissue CORRECT ANSWER-avidity what happens when amphoteric tissue (or dye) is placed in an acidic solvent? CORRECT ANSWER-becomes basic what happens when amphoteric tissue (or dye) is placed in a basic solvent? CORRECT ANSWER-becomes acidic what defines "how fast a dye stains" CORRECT ANSWER-vigour what type of sectionned tissue stains the fastest?...

Absorption - correct answers When tissue is penetrated by, or absorbs, a dye solution and becomes colored without any other change or chemical reaction occurring. An example is the absorption of oil red O or Sudan black B by fat Achromatic - correct answers Without color, or not easily stained. In microscopy, achromatic lenses are corrected for two colors, red and blue. This helps produce images free of chromatic aberrations Acid mucosubstances - correct answers a term used to include bot...

formalin CORRECT ANSWER-Which of the following pigments is birefringent? A. formalin B. bile C. lipofuscin D. melanin 8 - 10 micrometer CORRECT ANSWER-What micrometer thickness is preferred for the technique shown? skeletal CORRECT ANSWER-Muscle that histologically contains cytoplasmic cross-striations and has multiple nuclei located at the edge of the fibers is classified as: Von kossa CORRECT ANSWER-The technique shown below is most likely: Schmorl Stain CORRECT ANSWER-The tech...

Absorption - correct answers When tissue is penetrated by, or absorbs, a dye solution and becomes colored without any other change or chemical reaction occurring. An example is the absorption of oil red O or Sudan black B by fat Achromatic - correct answers Without color, or not easily stained. In microscopy, achromatic lenses are corrected for two colors, red and blue. This helps produce images free of chromatic aberrations Acid mucosubstances - correct answers a term used to include bot...

An example of an additive fixative is one that contains: CORRECT ANSWER-picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: CORRECT ANSWER-incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: CORRECT...