Dna electrophoresis - Study guides, Class notes & Summaries

H Biology: Gel Electrophoresis Questions (Quiz SG) Already Graded A What makes DNA think it’s in a race during gel electrophoresis? The electric current applied to the gel gives DNA a nudge, and since DNA is negatively charged, it dashes toward the positive side like it’s racing to the finish line! Why do the smaller DNA fragments win the "gel race"? Smaller fragments are like speedy sprinters—they easily slip through the tiny spaces in the gel, leaving the larger fragment...

Gel Electrophoresis (Honors Bio) Questions and Answers 100% Pass How do scientists visualize DNA after it has been separated by gel electrophoresis? Scientists use staining agents, such as ethidium bromide or SYBR Green, which bind to DNA and make it visible under UV light. What would happen if the gel concentration is too high in gel electrophoresis? If the gel concentration is too high, the pores would be too small, and larger DNA fragments would have difficulty moving through the g...

Gel electrophoresis Questions and Answers Graded A+ What kind of "track" does gel electrophoresis create for DNA? It creates a microscopic race track where DNA fragments can compete based on size, with smaller fragments sprinting ahead! If DNA fragments were athletes, how would they compete in gel electrophoresis? Smaller athletes (fragments) would zoom through the gel like sprinters, while larger ones lumber along, taking their sweet time. What’s the gel in gel electroph...

The Tools & Equipment of Gel Electrophoresis Questions and Answers Already Passed What is the role of the power supply in gel electrophoresis? The power supply provides the electric current that drives the movement of DNA through the gel. Why do we need a gel casting tray in gel electrophoresis? The gel casting tray holds the agarose gel as it solidifies, creating the platform for the separation of DNA fragments. What is the purpose of the comb in gel electrophoresis? The com...

Gel Electrophoresis Questions and Answers Already Passed What type of gel is typically used for separating nucleic acids? Agarose gel is typically used for separating nucleic acids like DNA and RNA. How does the size of DNA fragments influence their migration rate in gel electrophoresis? Smaller DNA fragments migrate faster through the gel than larger fragments due to less resistance in the gel matrix. What is the purpose of applying a dye to the gel before electrophoresis? Dyes ...

Gel Electrophoresis Questions and Answers Already Passed What property of DNA allows it to migrate towards the positive electrode during gel electrophoresis? DNA is negatively charged due to its phosphate backbone, causing it to move towards the positive electrode when an electric field is applied. In gel electrophoresis, what role does the gel matrix play in the separation of molecules? The gel matrix acts as a sieve, allowing smaller molecules to pass through more easily while re...

What techniques are used in molecular diagnosis? Correct Answer-Southern blot Dot blot/Reverse dot blot ***Polymerase chain reaction*** RT-PCR Mutation scanning: SSCP, DGGE, dHPLC Capillary electrophoresis Protein truncation test ***DNA sequencing*** Real-time PCR Invader assay In situ hybridization Microarrays What are the areas of application of molecular diagnostics? Correct Answer-Infectious Disease Neoplastic Disease ***Genetic Disease*** ***Identity Testing - fingerprint...

Biology- Gel Electrophoresis Questions and Answers Graded A+ How does the electric field strength affect the resolution of a gel? A stronger electric field increases the speed of migration but can reduce resolution; a balanced field strength is crucial for optimal separation. What precautions should be taken when using UV light to visualize DNA bands? Protective eyewear should be worn, and the exposure time should be minimized to avoid damaging the DNA and harming the eyes. What ...

Gel Electrophoresis Virtual Lab Questions and Answers Rated A+ What is the primary purpose of using a virtual lab for gel electrophoresis? A virtual lab allows students to simulate the gel electrophoresis process, helping them understand the technique without the need for physical materials and minimizing safety risks. How can you determine the size of DNA fragments after running gel electrophoresis in a virtual lab? By comparing the bands of the sample against a DNA ladder or marke...