The Tools & Equipment of Gel Electrophoresis Questions and Answers Already Passed
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The Tools & Equipment of Gel Electrophoresis
Institution
The Tools & Equipment Of Gel Electrophoresis
The Tools & Equipment of Gel
Electrophoresis Questions and Answers
Already Passed
What is the role of the power supply in gel electrophoresis?
The power supply provides the electric current that drives the movement of DNA through the
gel.
Why do we need a gel casting tray in gel elect...
The Tools & Equipment of Gel
Electrophoresis Questions and Answers
Already Passed
What is the role of the power supply in gel electrophoresis?
✔✔The power supply provides the electric current that drives the movement of DNA through the
gel.
Why do we need a gel casting tray in gel electrophoresis?
✔✔The gel casting tray holds the agarose gel as it solidifies, creating the platform for the
separation of DNA fragments.
What is the purpose of the comb in gel electrophoresis?
✔✔The comb creates wells in the gel where DNA samples can be loaded for separation.
Why is a micropipette essential for gel electrophoresis?
✔✔A micropipette is used to accurately measure and load small volumes of DNA samples into
the wells of the gel.
1
, What does the electrophoresis chamber do?
✔✔The electrophoresis chamber holds the gel and buffer, creating the environment for the DNA
to move when an electric current is applied.
Why do we use agarose gel in electrophoresis?
✔✔Agarose gel acts as a sieve, allowing DNA fragments to move at different speeds based on
their size during electrophoresis.
How does the buffer solution assist during the electrophoresis process?
✔✔The buffer solution conducts electricity and maintains the pH, ensuring the DNA moves
properly through the gel.
What tool is used to visualize DNA bands after electrophoresis?
✔✔A UV transilluminator is used to visualize the DNA bands, making the stained DNA glow
under ultraviolet light.
What is the purpose of loading dye in gel electrophoresis?
✔✔Loading dye helps track the progress of DNA through the gel and ensures the sample sinks
into the wells.
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