Gel Electrophoresis (Honors Bio) Questions and Answers 100% Pass
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Course
Gel Electrophoresis
Institution
Gel Electrophoresis
Gel Electrophoresis (Honors Bio)
Questions and Answers 100% Pass
How do scientists visualize DNA after it has been separated by gel electrophoresis?
Scientists use staining agents, such as ethidium bromide or SYBR Green, which bind to
DNA and make it visible under UV light.
What would happ...
Gel Electrophoresis (Honors Bio)
Questions and Answers 100% Pass
How do scientists visualize DNA after it has been separated by gel electrophoresis?
✔✔Scientists use staining agents, such as ethidium bromide or SYBR Green, which bind to
DNA and make it visible under UV light.
What would happen if the gel concentration is too high in gel electrophoresis?
✔✔If the gel concentration is too high, the pores would be too small, and larger DNA fragments
would have difficulty moving through the gel.
What role does the buffer play in gel electrophoresis?
✔✔The buffer maintains the pH and conducts the electric current, allowing the molecules to
move through the gel.
Why is it important to load the DNA samples carefully into the wells of the gel?
✔✔Careful loading ensures that the samples stay in the wells and are not mixed or spilled into
adjacent lanes.
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, What is the relationship between fragment size and migration distance in gel electrophoresis?
✔✔Smaller fragments migrate further through the gel, while larger fragments move more slowly
and travel shorter distances.
How is gel electrophoresis used in DNA fingerprinting?
✔✔It is used to separate and compare DNA fragments, creating a pattern unique to each
individual, like a fingerprint.
Why is it important to run a control sample in gel electrophoresis?
✔✔Running a control ensures that the experiment worked correctly and provides a baseline for
comparison.
What is the purpose of the loading dye in gel electrophoresis?
✔✔The loading dye helps visualize the movement of DNA samples and makes it easier to load
them into the wells.
What could cause smeared bands in a gel electrophoresis result?
✔✔Smeared bands could result from overloading the wells, running the gel too fast, or degraded
DNA.
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