SEA - Phages Lab Final UPDATED ACTUAL Questions and CORRECT Answers
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Course
SEA - Phages
Institution
SEA - Phages
SEA - Phages Lab Final UPDATED
ACTUAL Questions and CORRECT
Answers
*Explain the difference between sterile and aseptic.* - CORRECT ANSWER- sterile -
free from bacteria
aseptic - free from contamination caused by harmful bacteria, viruses, or other
microorganisms
*What is a plaque?* - COR...
SEA - Phages Lab Final UPDATED
ACTUAL Questions and CORRECT
Answers
*Explain the difference between sterile and aseptic.* - CORRECT ANSWER- ✔✔sterile -
free from bacteria
aseptic - free from contamination caused by harmful bacteria, viruses, or other
microorganisms
*What is a plaque?* - CORRECT ANSWER- ✔✔a clearing in a bacterial lawn resulting
from lysis by phages
*A phage genome that is dominant inside a bacterial cell's genome is called a* - CORRECT
ANSWER- ✔✔prophage
Two large biological molecules that compose a phage are: - CORRECT ANSWER-
✔✔proteins and nucleotides
describe the lytic and lysogenic cycle - CORRECT ANSWER- ✔✔bacteriophage infection
results in either a lytic or a lysogenic cycle:
1. LYTIC CYCLE results in the production of new progeny that cause the cell to lyse
2. LYSOGENIC CYCLE results in incorporation of the viral DNA into the bacterial DNA
where it remains inactive
-- a lysogenic cycle may progress into a lytic cycle
*a bacterium in which a phage has incorporated its genome is called a* - CORRECT
ANSWER- ✔✔lysogen
Why do we add the top agar to section 3 of the three-phase streak plate? How does this relate
to the fact that we shouldn't swirl the streak plate? - CORRECT ANSWER- ✔✔Section 3 is
where the most diluted phage exists. Top agar is added to section 3 so it will flow through
, sections 1 and 2 without crossing the different sections. We shouldn't swirl the streak plate
because this would cause the sections to cross and potential lysis of the entire plate.
Suppose you are lucky and isolate a phage from your soil sample that produces clear 2.0 mm
plaques on a M. smeg lawn. You want to do another streak plate but there is no more M. smeg
so you grab a culture tube of E. coli instead. Would you expect to see the same results after
48 hours? Why? - CORRECT ANSWER- ✔✔No -- only certain phages will grow with
certain host bacteria
When we filter-sterilize our sample with the 0.22 mm filters, why do we still expect to find
phages in the sample? - CORRECT ANSWER- ✔✔the phages are small enough to be filtered
through
Compare the plaque morphologies of lytic phages and temperate phages. - CORRECT
ANSWER- ✔✔lytic - clear plaques
temperate - cloudy ("turbid") plaques
bacteria are classified as - CORRECT ANSWER- ✔✔prokaryotes
*What is the purpose of adding nuclease to the DNA extraction?* - CORRECT ANSWER-
✔✔degrade bacterial DNA
*what is used to wash salts and proteins off column?* - CORRECT ANSWER-
✔✔isopropanol
bacteria are what type of organism? - CORRECT ANSWER- ✔✔prokaryote
compared to other groups, P. acnes phages are unusual because they have: - CORRECT
ANSWER- ✔✔less genetic diversity
In the DNA isolation procedure, what are you trying to isolate? - CORRECT ANSWER-
✔✔phage DNA
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