CSU LIFE 212 Exam 2 Actual Questions and CORRECT Answers
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Course
CSU LIFE 212
Institution
CSU LIFE 212
CSU LIFE 212 Exam 2 Actual Questions
and CORRECT Answers
bright field (What type of samples and what type of microscope) - CORRECT
ANSWER- background is brightly lit
stained, fixed samples (Light Microscope, compound)
Phase contrast (What type of samples and what type of microscope) - CORREC...
CSU LIFE 212 Exam 2 Actual Questions
and CORRECT Answers
bright field (What type of samples and what type of microscope) - CORRECT
ANSWER✔✔- background is brightly lit
stained, fixed samples (Light Microscope, compound)
Phase contrast (What type of samples and what type of microscope) - CORRECT
ANSWER✔✔- transparent, unstained samples-special lenses (annular stop turret in our lab
exercises)-background light is separated from the light diffraction pattern generated by the
specimen
(LM, comp)
Dark field (what type of microscope) - CORRECT ANSWER✔✔- background light is dark-
opaque disk inserted between light source and condensor (LM, comp)
Dissecting/stereoscope (what type of microscope) - CORRECT ANSWER✔✔- large working
distance-used for handling large specimens (LM)
Inverted microscope path - CORRECT ANSWER✔✔- objective lens below specimen
condensor above the specimen
used for tissue culture (LM)
Differential interference contrast - CORRECT ANSWER✔✔- separates polarized light into
two beams
the length of each optical path differs, which gives the appearance of a 3D relief
uses Nomarski optics (LM)
Fluorescence - CORRECT ANSWER✔✔- mercury or xenon light source
mirrors + excitation + emission filters allow only emitted light of a specific wavelength to be
seen (LM compound or inverted)
, Confocal - CORRECT ANSWER✔✔- a special type of fluorescence scope
software allows digital capture of image slices.
multiple images across the vertical Z axis are captured and reassembled in 3D reconstructions
reduces background fluorescence from unfocused parts of the specimen (LM)
Transmission Electron (what type of microscope) - CORRECT ANSWER✔✔- Uses high
energy electron beam to bombard thin specimen with electrons
specimen (~100 nMthick) with electrons
Dense parts of the specimen do not allow electrons to pass through the specimen.
Cytoplasmicareas appear clear.
Resolving Power = 3 to 10 Angstroms (A)
Best are 200000X human eye (electron microscope)
Electron Microscope
Scanning electron - CORRECT ANSWER✔✔- Used for un-sectioned specimens
Bombards the surface with electrons to reveal a 3D image.
Resolving power = 30-100A (EM)
Electron Microscope
1 Angstrom - CORRECT ANSWER✔✔- 0.1nm, 10 A = 1 nm
Atomic Force Microscopy/ Scanning Tunneling - CORRECT ANSWER✔✔- Used to deduce
overall shape of single molecules in solution.
Uses a minute probe to scan across a surface.
0.1 nm lateral resolution, 0.01 nm depth resolution (non-imaging)
electrical signal converted to image
X-Ray Crystallography - CORRECT ANSWER✔✔- Diffraction patterns are used to deduce
structure of individual proteins and complexes (NI)
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