Pglo - Study guides, Class notes & Summaries

What is the total volume of reagent in mL? - 0.250mL or 250 microliters pGlo plasmids, when taken up by a bacteria, will code for - a green fluorescence protein GFP Genetic transformation occurs when a cell takes up and expresses a new piece or foreign ____________, often a circular plasmid. - DNA What are 3 real-world links for the study/use of genetic GFP? - study of biological processes, observation of cell movement, use of GFP as a visual marker To genetically transform an entire organ...

what was function of incubation on ice - Incubation slows down the movement of the bilayer in cell. slows fluid cell membrane- fluid mosatic model what was the function of adding calcluim chloride - Addition of calcium chloride solutionthe calcium has a positive charger so it shields the negative phosphate in the nucliotide. You nutrelize it so it can go through the cell membrane because it is non polar. Adding the calcium chloride serves to shield the negative charge of DNA phosphate groups...

Lab Final Exam Questions with correct Answers define biofilm Is location in biofilms important? If so, why? -------------- define quorum sensing Heterogeneous - a lot of microbes living together Location in biofilm is important - most growth outside (more oxygen and food) *inside need different paths of fermentation Outside - fast growers Inside - slow growers Some dead Can have horizontal gene transfer Competent - capable of taking up DNA through transformation Bi...

Quiz lab of Biol228

Presents an in-depth overview of the calculations/math involved in making a gel for gel electrophoresis, gives important vocabulary/terms, provides reasons for gel, etc. while keeping the information brief.

You are working for the Sanger Institute and you have been asked to demonstrate basic DNA techniques to students visiting the facility. Carry out the following DNA techniques: • DNA Extraction • PCR Reaction and Electrophoresis • Restriction Fragment Length Polymorphism analysis and Electrophoresis • pGlo Genetic Transformtion Record your activities in your notebooks. For each practical you must include a description of the method, results, conclusions and photographs documenting your i...

Questions about transformation of bacteria to learn about their genetic material and growth patterns, as well as questions relating to epidemiology

Molecular Biology Lab for in class work and notes for during lab experiments.

Molecular Biology class work and in lab notes

Molecular Biology lab for the first three classes