Northern blotting - Study guides, Class notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as A. southern blotting B. northern blotting C. polymerase chain reaction D. DNA replication

MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

Tools used in biotechnology and genetic engineering - Answer-In Silico analysis, DNA microarray analysis, gel electrophoresis, PCR, and RFLP analysis. PCR - Answer-*Polymerase Chain Reaction* technique for quickly and easily making many copies of even a very small amount of DNA Reverse Transcriptase - Answer-enzyme that turns single-stranded RNA into double-stranded DNA Complementary DNA (cDNA) - Answer-DNA produced from an RNA template using reverse transcriptase. often used to clone ...

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

DAT Mnemonics Practice Exam Questions & Answers. Cell Cycle - CORRECT ANSWER Go Sam Go Make Cake G1 growth phase 1 of interphase S synthesis of interphase G2 growth phase 2 of interphase M mitosis of M phase C cytokinesis of M phase Nitrogenous bases - CORRECT ANSWER PUR As Gold = purines = adenine and guanine CUT the PY = pyrimidines = cytosine, uracil, thymine Types of blots - CORRECT ANSWER SNOW DROP southern blotting = DNA northern blotting = RNA western blotting ...

In a large nuclear war, nuclear detonations in cities and industrial areas would ignite enormous ____________ that would produce millions of tons of dense, black soot/smoke, which would be quickly lofted ________ and rise into the ______________. CORRECT ANSWER firestorms, above cloud level, stratosphere Drs. Robock and Toon have predicted that a nuclear war between India and Pakistan, in which a total of ____ atomic bombs are detonated in the largest cities of these two nations, would cause ...

Craniofacial Biology, Exam 1 with Practice Questions and Answers Name two specific purposes for cloning foreign DNA into plasmid vectors ANSWER protein drug production (like insulin) and gene therapy Briefly describe the cloning process ANSWER -restriction endonuclease digests and inserts the DNA into a vector with ligation - introduce newly ligated DNA into host cell (like E coli) -identify bacteria that have taken up the proper + vector Where to RE come from and what do Restriction ...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...