Microm 443 Study guides, Class notes & Summaries
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MICROM 443 Question and answers rated A+ 2024/2025
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MICROM 443 Question and answers rated A+ 2024/2025Genus Staphylococcus 
- Cells tend to form clusters, may be single, in pairs, or short chains 
- common skin microbiota 
- Generally hardy and not nutritionally fastidious 
- Facultative anaerobes (w/ O2 --> respire; without O2 --> ferment 
- Catalase positive 
 
 
Genus Streptococcus 
- Cells tend to form chains 
- Common oral microbiota 
- Nutritionally fastidious 
- Aerotolerent anaerobes/ obligate fermentors, some are caphophiles 
- Cat...
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Microm 443 Questions with complete solution 2024/2025
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Microm 443 Questions with complete solution 2024/2025Members of the family Enterobacteriaceae that are Lac+ (TSI = A/A) - correct answer E. coli, Enterobacter, Klebsiella and Citrobacter 50% of the time 
 
Members of the family Enterobacteriaceae that are Lac- (TSI = K/A) - correct answer Proteus, Shigella, Salmonella 
 
If you get a LIA slant that is R/Y (deamination) what two organisms should you think about? - correct answer Proteus and Providencia 
 
Members of the family Enterobact...
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MICROM 443 Questions with complete solution 2024/2025
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MICROM 443 Questions with complete solution 2024/2025 
What does a Methylene Blue stain show? What color? - correct answer metachromatic granules (volutin, polyphosphate) 
red/purple 
 
What does an Acid-Fast stain detect? - correct answer Mycobacterium species 
 
Why do incubate at 35C? - correct answer medically important bacteria grow at body temperature 
 
What is Blood agar? - correct answer rich media w/ red blood cells 
 
What is Chocolate agar? - correct answer lysed red blood ...
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MICROM 443 FINAL Question and answers already passed 2024/2025
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MICROM 443 FINAL Question and answers already passed 2024/2025Staphylococcus - correct answer - gram + 
- typically can isolate from skin 
- cocci in irregular clusters 
- catalase postive 
- non fastidious 
- facultative anaerobes 
- Can differentiate species by: coagulase test, novobiocin susceptibility, biochemical tests 
 
Staphylococcus aureus - correct answer - pathogen/opportunistic 
- some carry as Normal Microbiota of the nose 
- infections include: furuncles (boils), carb...
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MICROM 443 Question and answers rated A+ 2024/2025
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MICROM 443 Question and answers rated A+ 2024/2025Taxonomy 
Theory and practice of classification of individuals into groups. 
classification 
 
 
Phylogeny 
Study of evolution and ancestry of an organism. 
classification 
 
 
 
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Phenotype 
Morphology, biochemical capabilities; fatty acid (membrane and by-products), mycolic acid analysis. 
classification 
 
 
Genotype 
%G+C, DNA: DNA hybridization, sequence and whole genome sequence analysis. 
classification
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Microm 443 Midterm Question and answers correctly solved 2024/2025
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Microm 443 Midterm Question and answers correctly solved 2024/2025Staphylococcus aureus - correct answer Gram reaction: Gram positive cocci in clusters, tetrads and pairs 
Cell morphology: coccus 
Colony morphology: medium to large, white/creamy -> gold, possibly B-hemolytic 
Identification: B-hemolytic, catalase positive, most likely novobiocin sensitive, coagulase positive 
Normal Samples: carried in nose/nasal passage and skin of 10-40% of people 
Clinical samples: skin/hair follicle in...
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Microm 443 Final Exam Study Questions with complete solution 2024/2025
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Microm 443 Final Exam Study Questions with complete solution 2024/2025The space between two vertical lines is 1 millimeter (mm). How many 1-micron cocci could you line up end to end between the two lines. - correct answer 1000 
 
When observing a wet mount with the 40x objective, you see an organism whose width is approximately the same size as the pointer. The organism is likely a - correct answer yeast 
 
If during a gram stain you forgot to counter stain with safranin, what color would ...
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Microm 443 Quiz Question and answers rated A+ 2024/2025
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Microm 443 Quiz Question and answers rated A+ 2024/2025Anaerobic bacteria: Gram-neg rods - correct answer -Bacteroides fragilis group - cause abscesses and surgical infections (most common anaerobes recovered from clinical specimens; more resistant to penicillin and cephalosporins than other anaerobes; bile resistant) 
-Prevotella/Porphyromonas group - includes the black pigmented Pr. melaninogenica 
-Fusobacterium spp. - cause absession, aspiration pneumonia 
 
Anaerobic bacteria: Gram-pos r...
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MICROM 443 Quiz 5 Content Question and answers 100% correct 2024/2025
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MICROM 443 Quiz 5 Content Question and answers 100% correct 2024/2025What does the LIA test for? 
ability to deaminate lysine, carboxylate lysine, and/or produce H2S 
 
 
How is an LIA slant set up? 
The top is AEROBIC and tests for lysine DEAMINATION 
The bottom is ANAEROBIC and tests for lysine DECARBOXYLATION 
 
 
 
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Microm 443 Final Practical Question and answers correctly solved 2024/2025
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Microm 443 Final Practical Question and answers correctly solved 2024/2025Purpose of blood agar? - correct answer Detection hemolysis 
 
Chocolate agar - correct answer -Has NAD+ (factor V) added back to medium 
-Routinely used to grow Neisseria gonorrhea and Haemophius species 
 
Size of... 
Protozoa 
Yeast 
Bacteria 
Virus - correct answer 20-100 um 
4-10 um 
1um 
0.1 
 
Width of the pointer under .... 
10X ob 
40X ob 
100X ob - correct a
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