Chromotogrpahy - Study guides, Class notes & Summaries

Class notes CHEM 1127 Last document update:   ago

The type of interaction between the stationary phase, mobile phase, and substances in the mixture is the main element influencing the separation of molecules from one another. Small molecules like amino acids, carbohydrates, and fatty acids can be distinguished and identified very effectively using partition-based chromatography techniques. However, the separation of macromolecules like proteins and nucleic acids can be accomplished more effectively using affinity chromatography. Alcohol, esters...