Chelex extraction - Study guides, Class notes & Summaries

Spectrophotometry vs. Fluorometry - Answer Spectrophotometry Easy, but affected by protein/other contaminants Can't distinguish between DNA and RNA Fluorometry More steps, but can distinguish between DNA and RNA and contaminants do not affect Steps of Extraction with Chelating Resin - Answer 1. Mix Chelex Beads with specimen 2. Boil - Lyses cells 3. Spin beads and debris down 4. DNA in supernatant, Chelex at bottom. 5. Extracted by chloroform function of Chelex Beads - Ans...

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PTC is not a natural substance, but the ability to taste PTC is strongly correlated with - correct answer the ability to taste many plant-based bitter substances The gene responsible for tasting the PTC is known as TAS2R38, and is located on the - correct answer long arm of chromosome 7q35-36 The TAS2R38 membrane bound, receptor protein is expressed in - correct answer ...

Who is generally considered to be the originator of Forensic DNA typing A. Alec Jefferys B. Kary Mulis C. Bruce Budowle D. Colin Pitchfork E. John Butler - correct answer A. Alec Jefferys Which of the following is not a potential application of STR testing? A. Ascertaining ancestry B. Paternity testing C. Predicting future criminal behaviour D. Aiding disaster victim identification E. Identifying remains of soldiers killed in battle - correct answer C. Predicting future crim...

objective - correct answer characterize each student's ability to detect bitterness using a PTC test strip; assess if response to unsweetened grapefruit juice correlates with PTC test strip results; determine the density of taste bud-containing fungiform papillae (visual assay of the anterior aspect of student's tongue; correlate with PTC test strip and grapefruit juice responses); isolate DNA (saline mouth wash extraction), PCR amplify and (HaeIII...

Which of the following DNA extraction methods uses an ion-exchange resin suspension? a. organic b. Chelex c. FTA d. DNA IQ e. prepfiler - correct answer Chelex Which of the following do you think you could likely get DNA from? Select all that apply. a. urine in snow b. door knob c. bite out of a piece of cheesecake d. burned remains e. dandruff in a hat - correct answer all of them Why is it imperative that collected samples are thoroughly dried before sealing in an evidence ...

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