Bcmb406b - Study guides, Class notes & Summaries

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BCMB406B Lab 2 PCR Troubleshooting Questions with 100% Correct Verified answers ( A Grade)

BCMB406B Lab 2 PCR Troubleshooting Questions with 100% Correct Verified answers ( A Grade)
Exam (elaborations) • 2 pages • 2024

Reasons for sequence errors within PCR products - Answer Low fidelity of DNA polymerase, excess Mg2+ concentration, unbalanced dNTP concentrations, high number of cycles, UV-damaged DNA, or sequencing error Reasons for sequence error at termini of PCR products - Answer Problematic primer design, low primer quality, contaminating nucleases, UV-damaged DNA, or sequencing error