Bcmb 406b - Study guides, Class notes & Summaries
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BCMB 406B Midterm 1 Test-Questions with 100% Correct Verified answers
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Lab 1 - Exercise 1: Primer Analysis and PCR Trouble-Shooting - Answer Compared and 
contrasted two primer pairs to find the qualities that contribute to the likelihood of a 
given primer pair producing the desired product. 
- NetPrimer 
Lab 1 - Exercise 2: Primer Design from a Target Sequence - Answer 1. Nucleotide BLAST 
(BlastN): identify the sequence. 
--> indicate organism of origin, size of assigned DNA sequence, and the name of the 
protein it encodes.
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BCMB 406B Midterm 1 lab 1 Questions with 100% Correct Verified answers
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______ methodology allows for the study of complex 
*cellular regulation of genes 
*the role of specific amino acid residues in protein structure, catalytic function and 
ligand binding capacity 
*mechanisms involved in infectious and genetic disease - Answer Site directed 
mutagenesis
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BCMB 406B Midterm 1 study guide 2024
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What is the most important factor in a successful PCR reaction? - Well designed primers 
 
What can the theory of primer design also be applied to? - The use of oligonucleotides in other techniques that utilize annealing or formations of duplexes of nucleotides (probes in Southern blot, Northern Blot, ect.) 
 
What variables influence the success of a PCR reaction? - - quality of template DNA 
- cycling conditions (melting temp., duration) 
- specificity and stability of the PCR primers 
 
What ...
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BCMB 406B Midterm 1 lab 1 study guide
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PCR allows for: - amplification of minute amounts of template DNA 
can generate thousands or millions of copies of that sequence 
 
What is one of the most important parameters for a successful PCR? - well designed primers 
 
Name 3 variables that influence the success of a PCR reaction - 1. quality of template DNA 
2. cycling conditions 
3. specificity and stability of PCR primers 
 
The target sequence determines what criteria in primer design for PCR? - 1. primer length 
2. % GC content 
3. m...
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BCMB 406B Lab 1 Questions and Answers
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The success of a PCR reaction is mostly dependent on what? 
Answer> well designed primers 
Primer design can also be applied to what? 
Answer> - probes in Southern and Northern blots - DNA sequencing - 
microarray design 
What else can affect the success of a PCR reaction? 
Answer> - Quality of the template DNA 
- cycling conditions 
- Specificity and stability of the PCR primers 
What are key criteria in primer design? 
Answer> - Primer length 
- Percent GC content 
- Melting temper...
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BCMB 406B - Lab 1-Questions with 100% Correct Verified answers
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What describes a well defined PCR reaction? - Answer - template DNA is added in 
purified form 
- typically starts with 10^5 - 10^6 molecules of template DNA 
What are essential components to site directed mutagenesis? - Answer - DNA template 
- Primers 
- dNTPs 
- DNA polymerase
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BCMB 406B Lab 2 Questions and Answers
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Glycoside hydrolases 
Answer> Hydrolyze the glycosidic bond between 2 or more carbohydrates or 
carbohydrate-protein bonds 
Carbohydrate Binding Modules 
Answer> - Amino acid sequence within a carbohydrate-active enzyme with a 
discreet fold that has carbohydrate binding activity 
- Maintains the interaction of the enzyme with its substrate 
Vector NTI Advance 
Answer> - Allows you to plan your experiment and ensure that it results in the 
desired product 
Benefits of in silico 
Answ...
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BCMB 406B - Lab 1 exam questions and solutions
- Exam (elaborations) • 11 pages • 2024
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The success of a PCR reaction is mostly dependent on what? - well designed primers 
 
Primer design can also be applied to what? - - probes in Southern and Northern blots - DNA sequencing - microarray design 
 
What else can affect the success of a PCR reaction? - - Quality of the template DNA 
- cycling conditions 
- Specificity and stability of the PCR primers 
 
What are key criteria in primer design? - - Primer length 
- Percent GC content 
- Melting temperature 
- Sequence 
 
How are Primer...
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BCMB 406B - Lab 2 Test-Questions with 100% Correct Verified answers
- Exam (elaborations) • 7 pages • 2024
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Purpose of SDS-NaOH and Sodium Acetate in Alkaline lysis method? - Answer SDS- 
NaOH: achieves cell lysis 
- Chromosomal DNA remains in a high molecular weight form but is fully denatured 
Sodium Acetate Solution: neutralizes solution 
- Chromosomal DNA randomly base pairs and becomes insoluble 
- High salt concentration precipitates SDS-protein complexes and high molecular weight 
DNA
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BCMB 406B - Lab 2 exam
- Exam (elaborations) • 7 pages • 2024
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Glycoside hydrolases - Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds 
 
Carbohydrate Binding Modules - - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity 
- Maintains the interaction of the enzyme with its substrate 
 
Vector NTI Advance - - Allows you to plan your experiment and ensure that it results in the desired product 
 
Benefits of in silico - - Predicts restriction fragment s...
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