Bch 5413 Study guides, Class notes & Summaries

BCH 5413 TEST QUESTIONS WITH ANSWERS 2024...

RNA PROCESSING I & II -BCH 5413 EXAM LATEST UPDATE...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...

BCH 5413: 1-4 TEST QUESTIONS WITH ALL CORRECT ANSWERS Name 3 characteristic of Z-DNA - Answer-* Left-handed helix * 12bp per helical turn * Major groove flattened, nearly gone Fragile DNA is associated with - Answer-AT-rich cruciform To increase the melting temperature of DNA, you need to increase which two? a) bases b) salt c) phosphate d) AT e) coiling f) GC - Answer-b) and f) What does the genome consist of? - Answer-Chromosomes (linear DNA molecules with many genes) ...

Fragile DNA is associated with - Answer AT-rich cruciform To increase the melting temperature of DNA, you need to increase which two? a) bases b) salt c) phosphate d) AT e) coiling f) GC - Answer b) and f)

BCH 5413 EXAM PREP WITH COMPLETE SOLUTION

In the experiment described that supports DNA as the genetic material: a.Why does the mouse die if the researcher mixes dead pathogenic strain with live non-pathogenic strain bacteria and then inject it into the mouse? - ANSWER-The mouse dies because the DNA in the S strain transforms the R strain into becoming pathogenic b.What was the only scenario in which the mouse lives? Why? - ANSWER-The mouse lived when the DNA was destroyed because the S strain could not transform the R strain. Bas...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS Where in the genome are loxP sites located in a Cre/Lox model? - Answer-On either side of the gene that is to be knocked out In which organism was the CRISPR system discovered? - Answer-e. coli In the immunization phase, what happens to the foreign viral DNA? - Answer-Cleaved into pieces by cas complex and the fragments are called spacers. Some of the viral fragments will become a part of the sequence. What RNAs are present in the Cas9...

BCH 5413: 1-4 with Questions Solved 100% Correct