Affinity chromatography - Study guides, Class notes & Summaries

AAMC MCAT Practice Exam 2 Questions and Answers 2024 C/P: What expression gives the amount of light energy (in J per photon) that is converted to other forms between the fluorescence excitation and emission events? "intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 minutes" A) (6.62 × 10-34) × (3.0 × 108) B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) - 1 / (440 × 10-9)] D) (6.62 × 10...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

The polypeptide shown below needs to be separated from other polypeptides of similar size (at pH 7.0). What purification technique is best suited to isolate this peptide? A. Nickel affinity chromatography, since it contains a histidine residue that can bind metal. B. Size exclusion chromatography, since the polypeptide has some hydrophobic residues. C. Anion exchange chromatography, since the polypeptide has a net positive charge. D. Anion exchange chromatography, since the polypeptide ha...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis Ans- Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. ...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

What are the benefits of having smaller particles in HPLC? - correct answers high efficiency, higher plate number, higher pressure, shorter run time, better resolution due to uniform flow (reduced A-term, multiple pathway) what is plate height? - correct answers length of column required for 1 equilibration of solute between mobile and stationary phases What are the drawbacks of having smaller particles in HPLC? - correct answers lower detection limit, resistance to solvent flow, increased...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

Test Bank - Lehninger Principles of Biochemistry 8th Edition by David L. Nelson - Complete, Elaborated and Latest Test Bank. ALL Chapters (1-82) Included and Updated #BiochemistryFoundations #ProteinStructure #EnzymeFunction #NucleicAcids #LipidsAndMembranes #MetabolismIntro #Glycolysis #CitricAcidCycle #OxidativePhosphorylation #Photosynthesis #AminoAcidBiosynthesis #GeneExpression #BiochemistryTestBank #Bioenergetics #BiochemicalSignaling Chapter: 1. The Foundations of...