Steps of pcr - Study guides, Class notes & Summaries

ISSA Nutrition Exam Final 2023 WHAT IS GOOD NUTRITION? - Correct Answer-PROPERLY CONTROLS ENERGY BALANCE PROVIDE NUTRIENT DENSITY ACHIEVE HEALTH, BODY COMPOSITION AND PERFORMANCE GOALS HONEST AND OUTCOME BASE WHAT ARE THE 8 STEPS TO EFFECTIVE COACHING? - Correct Answer-PREPARE FOR CLEINT COLLECT PRELIMINARY CLIENT INFO EVALUATE CLEINT INFO AND STATE WHAT IT MEANS OFFER NUTRITIONAL SUGGESTIONS AND PLANS OFFER SUPPLEMENTAL SUGGESTIONS SET BEHAVIOR GOALS AND CREATE MONITOR STRTG MAKE N...

What is the goal of PCR? Correct Answer-To copy and amplify a DNA sequence. What are the requirements of PCR? Correct Answer-Template, primers, deoxyribosenucelotides, DNA polymerase, buffer What is are the general steps of PCR? Correct Answer- What is the purpose of Microarray/chip based hybridization? How do we accomplish this? Correct Answer-We want to get a GLOBAL profile of gene expression. We do this by determining the number of genes expressed in a cell and with how much abundanc...

After experiencing extreme fatigue and polyuria, a patient's basic metabolic panel is analyzed in the laboratory. The result of the glucose is too high for the instrument to read. The laboratorian performs a dilution using 0.25 mL of patient sample to 750 microliters of diluent. The result now reads 325 mg/dL. How should the technologist report this patient's glucose result? A. 325 mg/dL B. 1300 mg/dL C. 975 mg/dL D. 1625 mg/dL - B; The correct answer for this question is 1300 mg/dL. The ...

Males and Females are equally affected - Autosomal The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick ...

Catalyst - ANSWER-a substance that increases the rate of a chemical reaction without being consumed Enzyme - ANSWER-a molecule that increases the rate of a chemical reaction without being consumed (ex: protein) Activation energy - ANSWER-amount of energy required to convert a substrate into a product Active site - ANSWER-location on an enzyme that physically interacts with the substrate How do enzymes catalyze reactions? - ANSWER-1. substrate attaches to active site 2. activation en...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

How does BPA cause cancer? - it disrupts meiosis and mitosis causing breast and prostate cancer. 3 and 7 plastics may contain BPA What is a genetic fingerprint? how do you make one? - -Genetic Fingerprint: DNA uses the differences in base sequences to identify individuals. -DNA fingerprinting involves 3 tools: 1. PCR to get large amounts of DNA 2. Restriction digest with blunt ends to fragment the DNA with precision 3. Gel electrophoresis to separate and visualize the DNA fragments ...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Chapter 13 - DNA Purification, Agarose Gel Electrophoresis, and DNA Visualization - answer The genetic information of all living organisms is contained within the sequence of ____ ____ = Examples include: purines and pyrimidines = For most organisms, the genetic information is contained in nucleic acids of the deoxyribonucleic acid (DNA), or ribonucleic acid (RNA) in certain viruses - answernucleic acids ______ is a complete s...

Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR - 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: re...

Males and Females are equally affected - -Autosomal The process of copying DNA in the lab. - -Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - -What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - -What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - -What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers s...