Light microscopy - Study guides, Class notes & Summaries

Bio principles unit 2 brooks questions and answers graded A+ all organisms are made of cells and come from cells Cell theory When the cell size (INCREASES or DECREASES), the membrane gets smaller compared to cell volume. increases the study of cells Cytology "how can cork float" Took sections, looked under microscope and found that they had tiny chambers (aka cells) Robert Hooke Part of electromagnetic spectrum via sun, delivering energy that can be recorded as waveleng...

What are the different types of microscopy and staining and when are they used? CORRECT ANSWER Types of microscopy include: light microscopy- used primarily to get an idea of the form of the bacteria, used in combination with staining to get an idea of Gram positive and Gram negative protein. Green fluorescent is used to observe cell occurrences like binary fission and movement of proteins. Electron microscopy is very detailed at getting an idea of what is occurring inside and outside of the ce...

Microbiology Lab Midterm Questions and Answers Already Passed Compound microscope 1. Ocular-eyepiece lens (usually 10x) 2. Objective-nosepiece lenses (commonly 4x, 10x, 45x, and 100x) At what magnification do you use the oil immersion technique? 100x. Total Magnification = Magnification (Objective) x Magnification (Ocular) Important Parts of the Microscope Framework, Stage, and Lens System Framework Arm and base are the structural parts of the microscope which support the basic frame. Stage...

Major differences between plant and animal cells correct answers Animal: Only cell membrane Plastids Absent (heterotrophic) Vacuole One or few small Shape Irregular, no mechanical support In vitro regeneration: Single cell can not regenerate into a new organism Lysosomes Present Plant: Cell wall is present outside cell membrane, made of cellulose Plastids Present, perform photosynthesis (autotrophic) Large vacuole for storage (about 90%) of cell space in mature cells Fixed shape...

BIO 353 test 1 multiple choice -ASU questions & answers 2024 A student in a cellular biology laboratory is faced with the task of detecting protein X in muscle cells taken from a rat. The tissue is required to be live while under analysis. Which type of microscopy should be employed? a) Phase contrast light microscopy b) Transmission electron microscopy c) Scanning electron microscopy d) Fluorescence microscopy e) None of the above - ANSWER-d) Fluorescence microscopy Which...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

Electron beams can not be used to resolve images of objects. T/F? Correct Answer-False In all types of electron microscopy, electrons pass through the specimen. Correct Answer-False This type of light microscopy can be used to view very thin cells or flagella. It does that by using this property of light. Correct Answer-Dark field; Light scattering In this type of microscopy, light is delayed by passing through the sample and so objects look as if they are out of phase. It employs this ...

Last Universal Common Ancestor (LUCA) CORRECT ANSWER Was a microbe of unknown type that lived about 4 billion years ago; the origin of all organisms. What is 16s small unit rRNA used for? CORRECT ANSWER It's used to study construct phylogenetic trees of organisms because it's highly conserved and hasn't changed much over time. Antonie von Leeuwenhoek CORRECT ANSWER Father of microscopy in 1620 Increased surface area like folded membranes CORRECT ANSWER How do larger cells maximize di...

Spectroscopy Methods correct answers- Fluorescence Microscopy - super resolution microscopy - flow cytometry / mass cytometry Ligand binding can be tested by correct answers- equilibrium dialysis - Fluorescence (anisotropy) - FRET - SPR Enzyme kinetics can be tested by correct answers- UV-Vis spectroscopy - Fluorescence - FRET Modes of inhibition correct answers UV-Vis spectroscopy correct answers- light absorption by chromophores - measure the amount of l...

Portage Learning: Microbiology Module 3 Already Graded A Define the measurements micrometer and nanometer. A micrometer (μm) is defined as being one-millionth of a meter and is commonly designated at 10-6 meters. A nanometer (nm) equals 10-9 m or one-billionth of a meter. What are the two critical factors that influence your ability to see an object? Resolution and contrast. Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low r...