After 1995 - Study guides, Class notes & Summaries
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED 
E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY 
Lee Jia Yi 
 (12) 
Abstract 
In this study, a recombinant histidine-tagged 
green fluorescence protein(His6-GFP) was 
produced by E.coli XL1 cells followed by 
immobilized metal affinity chromatography 
(IMAC) using Nickel-agarose beads for 
purification. The GFP template was first 
amplified from pE-GFP vector using 
polymerase chain reaction (PCR). The primers 
c...
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IOP4863 Assignment 5 (COMPLETE ANSWERS) 2024 (688200) - DUE 19 September 2024
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IOP4863 Assignment 5 (COMPLETE ANSWERS) 2024 (688200) - DUE 19 September 2024...Detailed work, solutions, memos, notes, and explanations....PART A: PERSONNEL PSYCHOLOGY Generally, South African labour laws prohibit unfair discrimination. The Labour Relations Act 66 of 1995 (as amended) and the Employment Equity Act 55 of 1998 (as amended) are explicit on issues of unfair discrimination. Despite this regulatory framework, debates around the issues of fairness remain intense. Fairness in personnel...
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ICH4801 Assignment 4 (COMPLETE ANSWERS) 2023 (642913) - DUE 31 October 2023
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ICH4801 Assignment 4 (COMPLETE ANSWERS) 2023 () - DUE 31 October 2023 100% TRUSTED workings, explanations and solutions. for assistance SECTION A: HISTORY OF EDUCATION [50] Question 1 Section A is based on the given extract from chapter 2 of the prescribed textbook (Seroto, Davids & Wolhuter 2020). Read the extract and then answer the questions that follow. Geographical focus of research and of authorship Scholars, analysts, progressive scholars and academics in all education sciences and beyond...
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LEG2601 Assignment 2 (ANSWERS) Semester 1 2024 - DISTINCTION GUARANTEED
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LEG2601 Assignment 2 (ANSWERS) Semester 1 2024 - DISTINCTION GUARANTEED. Answers, guidelines, workings and references..... .... Question 
1 
Complete 
Marked out of 1.00 
Question 
2 
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QUIZ 
… is an example of a heritage resource protected under the National Heritage Resources Act 25 of 1999. 
a. 
Great-grandmother’s vase. 
b. 
A collection of South African 1 cent coins (no longer in circulation). 
c. 
Any gravesite. 
d. 
A building older than 60 years. 
A number...
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ICH4801 Assignment 4 (COMPLETE ANSWERS) 2024 (390113) - DUE 20 August 2024 ;100% TRUSTED workings, explanations and solutions
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ICH4801 Assignment 4 (COMPLETE ANSWERS) 2024 (390113) - DUE 20 August 2024 ;100% TRUSTED workings, explanations and solutions. ...... Question 1 Section A is based on the extract from chapter 2 of the prescribed textbook (Seroto, Davids & Wolhuter 2020). Read the extract and then answer the questions that follow. Geographical focus of research and of authorship Scholars, analysts, progressive scholars and academics in all education sciences and beyond, have expressed concern that the corpus of s...
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LRM3702 Assignment 8 2023 (ANSWERS)
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LRM3702 Assignment 8 2023 (ANSWERS) 
 
QUESTIONS ANSWERED: 
QUESTION 1 
Read the following news report and answer questions 1.1 and 1.2 that follows. 
SEVERAL HOSPITALS HIT HARD BY PUBLIC SECTOR STRIKE 
The ongoing public sector strike has had severe consequences for several major hospitals in three 
provinces. On Tuesday, doctors and nurses across these provinces faced significant challenges due to 
the strike. Protesters blocked access to the hospitals, making it extremely difficult for heal...
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MRL3701 EXAM PACK 2024
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MRL3701 
INSOLVENCY LAW 
PAST EXAM PACK QUESTIONS AND ANSWERS 
SUMMARISED NOTES 
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MRL 3701 
OCTOBER/NOVEMBER 2018 
QUESTION 1 
a) Inability to pay debts is, at most merely evidence of insolvency. (page 3) 
b) Brenda’s application for voluntary surrender must be set out the causes of her 
insolvency in some detail to enable the court to determine whether the 
application is ......................... 
c) Christopher commits an act of insolvency by depar...
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NR533 Week 7 Assignment: Business Plan Assignment (2 VERSIONS).
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NR533 Week 7 Assignment: Business Plan Assignment (2 VERSIONS). 
Business Plan 
Student Name 
Chamberlain School of Nursing 
NR533 Financial Management in Healthcare Organizations 
February 21, 2021 
BUSINESS PLAN 2 
Business Plan 
A business plan is a convincing written document that clearly outlines its goals, purpose, 
and objectives which the creator is attempting to be obtained. Developing a business plan per 
Johnson & Garvin 2017, and Sherman, 2016 is written in a timeline as a set of ev...
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EPA 608 / epa 608 universal universal With correct Answers
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EPA 608 / epa 608 universal universal With correct Answers 
Refrigeration technicians who violate the Clean Air Act can: - - ANSWER-Lose their EPA certification, be fined, be required to appear in Federal court. 
After 1995, any supplies of CFC refrigerants to service existing units in the USA can only come from: - - ANSWER-recovery and recycling.
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EPA 608 Certification Exam with 100% Correct Answers 2024 / 2025
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What date was the sale of CFC's & HCFC's restricted to technicians certified in 
refrigerant recovery? - Correct answer-November 14, 1994 
Section 608 of the Federal Clean Air Act requires technicians to what? - Correct 
answer-Recover all refrigerants. 
The objective of the Clean Air Act is to protect what? - Correct answer-The earth's 
stratosphere. 
HFC's - Correct answer-HydroFlouroCarbon's 
CFC's - Correct answer-ChloroFluoroCarbon's 
ODP - Correct answer-Ozone Depletion Potential 
Which in...
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