Tsi agar slant Study guides, Class notes & Summaries
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Microbiology Practical Review- Carbohydrate Catabolism Exam Questions with Latest Update
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Types of Tests (4) - ANSWER-TSI 
 
Citrate 
 
Carbohydrate Fermentation Test w/ Durham Tubes 
 
MR/VP 
 
TSI Test - ANSWER-Triple Sugar Iron Test (slant) 
 
both a streak and a slant 
 
Citrate test - ANSWER-typically only a streak is done but both a streak and slant may be performed 
 
MR/VP test - ANSWER-Methyl Red 
Voges- Proskauer 
 
TSI Composition - ANSWER-Basic T Soy Agar plus: 
 
1. glucose, lactose, & sucrose. Lac and Suc are 10x the conc. of glucose 
 
2. Phenol red (pH indicator) pH 6...
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BIOD 171 lab exams 1-9 (GRADED A) Questions and Answer solutions | Portage Learning
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BIOD 171 lab exams 1-9 LAB 1 Answer the following questions 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is the minimum temp erature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. Hot steam is the best choic...
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Biochemical Test - Triple Sugar Iron (TSI) Questions with Answers,100% CORRECT
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Biochemical Test - Triple Sugar Iron (TSI) Questions with Answers 
 
 
Tests for... - CORRECT ANSWER carbohydrate fermentation, gas production, & hydrogen sulfide production 
 
TSI slant - CORRECT ANSWER contains agar, pH indicator (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, & sodium thiosulfate & ferrous sulfate; as media cools after autoclaving, test tubes placed at slanted angle - provides oxygen-rich surface (aerobic) & oxygen-depleted butt (anaerobic) 
 
how the test works - CORRECT...
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MICROBIOLOGY LAB PRACTICAL #2 REVIEW QUESTIONS WITH COMPLETE SOLUTIONS
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TSI Agar correct answer: *stab and drag 
*yellow= acid production/ fermentaion of the sugars in the slant/butt. 
*red= negative 
*black= H2S production (salmonella or proteus) 
 
what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates that the amino acid tryptophan has been degraded into indole. 
 
Methyl red test results? correct answer: red= lots of acid (PH of 4) normally yellow=acidic 
 
Simmons citrate correct answer: to see i...
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Triple Sugar Iron Test Questions With Complete Solutions
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For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. 
 
How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. 
 
Why is there more lactose and sucrose in TSI agar than glucose? correct answer: In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will also allow for greater acid production. 
 
TEST RESULTS: red slant/r...
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SALMONELLA NOTES QUESTIONS AND ANSWER 2023 GRADED A+ UPDATE
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SALMONELLA NOTES QUESTIONS AND ANSWER 
2023 GRADED A+ UPDATE 
Salmonella 
ANSWER = Genus with over 2200 pathogenic serotypes 
Serotype classification 
ANSWER = Based on Kauffman-White system 
Gastroenteritis 
ANSWER = Moderate to severe illness caused by Salmonella 
Transmission 
ANSWER = Contaminated water and undercooked food, especially chicken 
Salmonella typhi 
ANSWER = Causes typhoid fever 
Salmonella Subgroup I 
ANSWER = Includes Salmonella typhi and Salmonella paratyphi 
Suspect Salmonel...
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bio 171 Lab 7 Notebook
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bio 171 Lab 7 Notebook. Detailed working, solutions, memo, notes & explanations. Lab 7 Notebook 
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Title: PL#7 Secondary Assays for Bacterial Characterization 
Objective: This lab identifies the profile of bacterial populations that are based on additional 
biochemical approaches to best determine the growth and metabolic characteristics. 
Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown) 
tryptophan into indole. 
Procedure: Test #1 
1...
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Triple Sugar Iron Test Exam With Guaranteed Accurate Answers
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what is the purpose of the triple sugar iron test? - correct answer -the TSI test is designed to differentiate among the different groups or genera of the Enterobacteriaceae, which are all gram-negative bacilli capable of fermenting glucose with the production of acid, and to distinguish the Enterobacteriacase from other gram-negative intestinal bacilli. 
 
What is the enterobacteriacase? - correct answer -all gram negative bacilli capable of fermenting glucose with the production of acid. 
 
wh...
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BIOD 171 Lab 7 notebook portage 2023
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Lab 7 Notebook 
Title: SK07 Secondary Assays for Bacterial Characterization 
Objective: To profile a bacterial populations based on additional biochemical approaches to 
better determine growth/metabolic characteristics 
Procedure: 
Indole Test (Test #1) 
1. Using a sterile look pick an isolated colony from BAP 
2. Smear directly onto the reaction area of the slide 
3. Examine test area for color change within 20 seconds 
Indole Test (Test #2) 
1. Using a sterile loop pick an isolated colony fro...
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BOC Microbiology Revision Exam Questions With Guaranteed Pass Solutions.
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Biochemically, the Enterobacteriaceae are gram negative rods that: - Answer Ferment glucose, reduce nitrate to nitrite, and are oxidase negative. 
 
THe ortho-nitrophenyl-Beta-galactopyranoside (ONPG) test is most useful when differentiating: - Answer Shigella spp. from some strains of Escherichia coli 
 
The Voges-Proskauer (VP) test detects which end product of glucose fermentation? - Answer Acetoin 
 
At which pH does the methyl red(MR) test become positive? - Answer pH of 4.5 
 
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