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MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

FL 5 AAMC MCAT Exam Questions and Answers 100% Verified & Updated What is the chemical structure of a component found in four of the five cofactors used by Na+-NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has...

Bio 315 Exam 1 Questions and Answers 100% Pass Cytology - Correct Answer ️️ -focuses mainly on cellular structure and emphasizes optical techniques Biochemistry - Correct Answer ️️ -focuses on cellular structure and function Genetics - Correct Answer ️️ -focuses on information flow and heredity and includes sequencing of the entire genome (all of the DNA) in numerous organisms Robert Hooke - Correct Answer ️️ -Discovered cells 30x magnification -termed cells Cellula -drew...

Medical Biochemistry, UCF questions and answers latest top score. Alanine - correct answers.Ala, A Arginine - correct answers.Arg, R Asparagine - correct answers.Asn, N Aspartic Acid - correct answers.Asp, D Experimental techniques: PCR - correct answers.-can amplify small amounts of DNA Experimental techniques: Western blot - correct answers.-measures protein levels in a sample using antibodies Experimental techniques: Southern blot - correct answers.-de...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample at A260 = 1...

ACEDS/ACTUAL EXAM LATEST VERSION WITH VERIFIED QUESTIONS AND ANSWERS GRADED A+ Henderson-Hasselbach Equation - ANS--pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANS--Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Collagen - ANS--Repeating sequence of Gly-X-Pro. 3 stranded "coiled coil". Contains gly core. Myoglobin 4° Structure - ANS--Symmetric homodimer, Salting Out (Purification) - ANS--Changes...

ACS Biochemistry Final Exam Questions and Correct Solutions 2024 pKa (chapter 3) - Answer -measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - Answer -compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - Answer -dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - Answer -the ch...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...