Paraffin sections - Study guides, Class notes & Summaries

HISTOTECHNOLOGYArt and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. Histotechnology Used to view slides not seen by the naked eye microscope Used by pathologist to view slides to identify a disease or process. microscope Used by histotechnologist to view slides FOR QUALITY CONTROL microscope Basic instrument used in microtomy (the process by which processed tissue, most common...

What is the purpose of embedding? - Answer hold tissue; orient tissue Why do we want the tissue held? - Answer so we can cut very thin sections and it has more stability Why do we orient tissue? - Answer so that the view the pathologist has is optimal for diagnosis List the two types of Embedding media - Answer Water-soluble and water-insoluble List examples of water-soluble media - Answer Carbowax, gelatin, agar, OCT What is carbowax used for? - Answer CNS Wh...

Histotechnology- Microtomy1. If there is venetian blind pattern in the section, a possible remedy is to: - correct answers Tighten all screws 2. To decrease the effect of static electricity: - correct answers ground microtome or breathe on it 3. To remedy crooked ribbon formation: - correct answers trim block edges parallel 4. If the specimen is mushy-appearing and sectioning is difficult: - correct answers a. melt block, reprocess & re-embed 6. A scratching noise during sectioning c...

MLPAO Exam Questions and Answers Already Graded A Which of the following is the counterstain for the gram stain method? - crystal violet - safranin - acetone-alcohol - iodine - gentian-violet safranin Most fixatives act by: - precipitating calcium ions in cells - precipitating cell wall - precipitating carbohydrates in cells - precipitating enzymes in the cells - none of the above precipitating cell wall A Westergren tube is used for which test procedure? - prothrombin time - hematocrit - pla...

Histotechnology test 2What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 degr...

Explain the steps involved in tissue processing - ANSWER-1. Surgery 2. Fixation 3. Grossing 4. Fixation 5. Processing 6. Embedding 7. Staining 8. Cover-slipping Discuss the process of fixation - ANSWER-Stabilization of proteins to prevent autolysis and putrefaction. -Maintain proper relationship between cells and extracellular material (keep as "life-life" as possible) -15-20 times volume fixative of tissue -10% NBF most common fixative Describe the goal of tissue proces...

MLPAO Test Prep ~ Histology and Cytology Latest Update Rated A Histology Definition Study of the structure of tissues Histology: Types of specimens received Surgical procedures, biopsies, autopsies Histology: Surgical specimens include Whole or part of an organ, cysts, tumors Histology: Biopsy specimens include Removal of a representative sample (through needle aspiration, surgery, or endoscopy) Histology: Autopsy specimens include Removal of tissue from a deceased person (to analyze the cau...

1. If there is venetian blind pattern in the section, a possible remedy is to: CORRECT ANSWER-Tighten all screws 2. To decrease the effect of static electricity: CORRECT ANSWER-ground microtome or breathe on it 3. To remedy crooked ribbon formation: CORRECT ANSWER-trim block edges parallel 4. If the specimen is mushy-appearing and sectioning is difficult: CORRECT ANSWER-a. melt block, reprocess & re-embed 6. A scratching noise during sectioning can be caused by: CORRECT ANSWER-tissue...

Histotechnology-- Question and answers 100% correct 2023/2024Microscope - correct answers Used to for the magnification of the tissues and cells. Microtome - correct answers Used for the cutting of the wax and tissues. Block holder (chuck) - correct answers Holds the tissue in position. Knife and Knife carrier - correct answers For actual cutting of tissue sections. Pawl, Ratcher Feed Wheel & Adjustment screw - correct answers Lines up the tissue block in proper position with knife, ...

HTL Histotechnology Exam 1The overall magnification of a microscope is achieved by what two values? - correct answers objective lens x eyepiece lens What is the magnification of scanning or low power? - correct answers 2.5x to 4.0x What is the magnification of intermediate power? - correct answers 10x to 20x What is the magnification of high power? - correct answers 40x What is the magnification of high dry? - correct answers 100x What is the magnification of oil immersion? - corr...