Northern blotting - Study guides, Class notes & Summaries

©THEBRIGHT EXAM STUDY SOLUTIONS 8/21/2024 8:36 PM MCB3020 Exam 3 Questions With Correct Answers Reverse transcriptase process - answer1. poly-T primer to single-strand RNA 2. reverse transcriptase to produce DNA-RNA hybrid 3. enzyme RNaseH to cut RNA and regenerate RNA primers 4, DNA polymerase and ligase synthesizes new complementary DNA strand cloning vector - answerallows for transfer of gene of interest to a host organism; allows for the making of recombinant DNA good cloning vecto...

AAMC full length 2, EXAM Overviews 2023 Update amide linking is peptide on the carboxyl side - ANS-so amide linkage involving the N atom in AMC used to covalently attach flurophore to the peptide what expression gives the amount of light energy (J/photon) that is converted to other forms between the fluorescence excitation and emission events? - ANS-E=hf = hc/lambda, here we see that excitation is at 360 nm but observation is at 440 nm. so you have E = (6.62 x 10^-34)(3x 10^8)x (1/360...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...

Define biotechnology - Answer- The use of technology to improve living organism and processes for the bennifit of man Briefly state the main difference of modern from classical biotechnology - Answer- Modern BTEC involves recombinant DNA technology Selective breeding is an early form of classical biotech, where there is deliberate crossing of parent organisms with favorable traits. Name two vegetables that are products of selective breeding from one common ancestor that were shown in the l...

DAT Practice Test Questions and Answers 2024/2025 apoptosis - CORRECT ANSWER-mediated cell death Change salt concentrations or pH - CORRECT ANSWER-how to remove a peripheral protein add a detergent - CORRECT ANSWER-how to remove an integral protein a light centrifuge - CORRECT ANSWER-precipitate out cells or nuclei a heavy centrifuge - CORRECT ANSWER-extract small, soluble proteins ammonia, methane, water, hydrogen - CORRECT ANSWER-the Miller-Urey experiment used... muscle cells - C...

MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DN...

What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel the size of the DNA fragment What factors affec...

Biochemistry ACS Review Questions With 100% Complete Solutions A codon is present in _______. An anticodon is found in _______. The 5' - AUC - 3' codon, will base pair with the 5' - _______ - 3' anticodon. A. mRNA, tRNA, UAG B. mRNA, tRNA, GAT C. DNA, mRNA, GAU D. mRNA, tRNA, GAU E. DNA, mRNA, CUA - Answer-D. mRNA, tRNA, GAU Anticodon is reversed because it is running 5'-3 /.A researcher wants to determine if cancer cells express protein X. Which technique would be the mos...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...