Fmoc chemical synthesis - Study guides, Class notes & Summaries
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ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT
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ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT 
 
Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) 
 
FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
 
Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
 
Size-Exc...
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Med Chem II Exam #1 With 100% Correct Answers 2024
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Med Chem II Exam #1 With 100% Correct 
Answers 2024 
What side of the growing peptide chain does coupling with DIC add to in 
solution? 
N-terminus 
Protecting group used with an acid reagent 
Boc 
Protecting group used with a base reagent 
Fmoc 
Protecting group used with Pd/C, H2 reagent 
Cbz 
Advantages of solid phase peptide synthesis (3) 
1. Can drive reactions to completion by using huge excesses of reagents 
2. Easy purification after each chemical step 
3. Widely diverse selection of res...
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ACS BIOCHEMISTRY EXAM Questions and Correct Answers the Latest Update
- Exam (elaborations) • 34 pages • 2024
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Henderson-Hasselbach Equation 
 pH = pKa + log ([A-] / [HA]) 
FMOC Chemical Synthesis 
 Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is 
used as a protecting group on the N-terminus. 
Salting Out (Purification) 
 Changes soluble protein to solid precipitate. Protein precipitates when the charges 
on the protein match the charges in the solution. 
Size-Exclusion Chromatography 
 Separates sample based on size with smaller molecules eluting later. 
Ion-Exchange Chr...
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ACS BIOCHEMISTRY EXAM Questions and Correct Answers the Latest Update
- Exam (elaborations) • 38 pages • 2024
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Henderson-Hasselbach Equation 
 pH = pKa + log ([A-] / [HA]) 
FMOC Chemical Synthesis 
 Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is 
used as a protecting group on the N-terminus. 
Salting Out (Purification) 
 Changes soluble protein to solid precipitate. Protein precipitates when the charges 
on the protein match the charges in the solution. 
Size-Exclusion Chromatography 
 Separates sample based on size with smaller molecules eluting later. 
Ion-Exchange Chr...
-
ACS BIOCHEMISTRY EXAM 200+ QUESTIONS WITH VERIFIED ANSWERS BY EXPERTS 2023 LATEST UPDATE.
- Exam (elaborations) • 19 pages • 2023
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ACS BIOCHEMISTRY EXAM 200+ 
QUESTIONS WITH VERIFIED 
ANSWERS BY EXPERTS 2023 
LATEST UPDATE. 
1. Henderson-Hasselbach Equation: pH = pKa + log ([A-] / [HA]) 
2. FMOC Chemical Synthesis: Used in synthesis of a growing amino acid chain 
to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
3. Salting Out (Purification): Changes soluble protein to solid precipitate. Protein 
precipitates when the charges on the protein match the charges in the solution. 
4. Size-Exclusion Ch...
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ACS BIOCHEMISTRY EXAM WITH 100% CORRECT ANSWERS
- Exam (elaborations) • 18 pages • 2024
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ACS BIOCHEMISTRY EXAM WITH 100% 
CORRECT ANSWERS 
Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) 
FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a 
polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein 
precipitates when the charges on the protein match the charges in the solution. 
Size-Exclusion Chromatography - answerSeparates samp...
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ACS BIOCHEMISTRY EXAM Terms in this set (252) Henderson-Hasselbach Equation	pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis	Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-te
- Exam (elaborations) • 5 pages • 2024
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ACS BIOCHEMISTRY EXAM 
 
 
 
 
 
 
Terms in this set (252) 
 
Henderson-Hasselbach Equation	pH = pKa + log ([A-] / [HA]) 
FMOC Chemical Synthesis	Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
Salting Out (Purification)	Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
Size-Exclusion Chromatography	Separates sample based on size with ...
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ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024
- Exam (elaborations) • 18 pages • 2023
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COMPLETE SOLUTIONS LATEST 2023/2024 
 
 
 
 
 
Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) 
 
FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 
 
Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 
 
Size-Exclusion Chromatography - ANSWER Separate...
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ACS BIOCHEMISTRY EXAM WITH CORRECT DETAILED ANSWERS 2023
- Exam (elaborations) • 18 pages • 2023
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ACS BIOCHEMISTRY EXAM WITH CORRECT DETAILED ANSWERS 2023
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ACS BIOCHEMISTRY EXAM Questions Answers Graded A 100 VERIFIED Latest Update 2023
- Exam (elaborations) • 31 pages • 2024
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ACS BIOCHEMISTRY EXAM | Questions & Answers (Graded A+) |100% VERIFIED Latest Update 2023 
Henderson-Hasselbach Equation 
FMOC Chemical Synthesis 
Salting Out (Purification) 
Size-Exclusion Chromatography 
Ion-Exchange Chromatography 
Hydrophobic/Reverse Phase Chromatography 
Affinity Chromatography 
SDS-PAGE 
SDS 
Isoelectric Focusing 
FDNB (1-fluoro-2,3-dinitrobenzene) 
DTT (dithiothreitol) 
Iodoacetate 
Homologs 
Orthologs 
Paralogs 
Ramachandran Plot 
Glycine Ramachandran Plot 
Proline Ramac...
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