Crispr cas9 - Study guides, Class notes & Summaries

Causes of chromosomal aberrations - errors in chromosome segregation (causes whole chromosome aneuploidy) and/or DNA damage repair (causes structural variation) Anneal - the ability of two complementary nucleic acids to align in an opposing orientation to allow the nucleotide bases of one strand to form hydrogen bonds with the nucleotide bases of the complementary strand; used in microarrays to bind DNA and show fluorescence Absolute risk - A person's chances of developing a disease or dis...

CRISPR Cas-9 Questions UPDATED Exam Questions and CORRECT Answers How many nucleotides long is the guiding region of the sgRNA? - Correct ANSWER- 20 nucleotides Does the sgRNA bind to the PAM? - Correct ANSWER- Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - Correct ANSWERIt cuts the DNA 3 nucleotides upstream of the PAM

LIFESCI 2G03 Term Test 1 Questions and Answers Fall 2024 with complete Solution; McMaster University Multiple Choice Questions Choose the one alternative that best completes the statement or answers the question. 1. Assume that a wild-type sequence is 5' AGCCTAC 3'. Which sequence could be produced by a transversion? A. 5' ATCCTAC 3' B. 5' AGCCCAC 3' C. 5' AGCCGCCGCCGCCTAC 3' D. 5' AGCCTGC 3' 2. In an organism DNA, an (A) base is changed to a (C) and the error is ...

In dit document staat alle informatie die is verteld tijdens zowel de theorie lectures als the applicatie guest lectures. Het zou meer dan genoeg moeten zijn voor een goed cijfer op het tentamen.

In this document: Chapter 10 Eukaryotic Chromosome Abnormalities. Chromosome Aberrations and Transposition. Analysis of Gene Function by Forward and Reverse Genetics. Application of CRISPR-Cas9. Gene Therapy for Mice with Sickle Cell Disease. Developmental Genetics. Drosophilia Life Cycle.

CRISPR-Cas9 Final Practice Exam Questions 2024.What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of re...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...

CRISPR-Cas9 Complete Study Guide Practice Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kin...