Column chromatography - Study guides, Class notes & Summaries

CHEM 209 Quiz 3 Latest Version with Complete Solutions What variables are typically plotted on the x- and y-axes of a chromatogram? x-axis: time, y-axis: response What variables are typically plotted on the x- and y-axes of a calibration curve? x-axis: concentration, y-axis: peak area What term describes the time it takes for a molecule to move through the column? retention time What term describes the material coating the inside of the column in chromatography? stationary...

carrier gas - correct answers MP gase in GC cold trapping - correct answers Splitless gas chromatography injection technique in which solute is condensed far below its boiling point in a narrow band at the start of the column. electron capture detector - correct answers gas chromatography detector that is particularly sensitive to compounds with halogen atoms, nitro groups, and other groups with high electron affinity flame ionization dectector - correct answers gas chromatography detec...

CHEM 209 Quiz 3 Latest Update with Verified Solutions Provide a diagram illustrating the key components of a GC instrument. Answer: Reservoir for Mobile Phase (pressurized helium cylinder) -> Oven -> Sample Injector (micro-syringe) -> Stationary Phase (column) -> Detector (thermal conductor or flame ionization detector) -> Readout (computer) What variables are typically plotted on the x- and y- axes of (a) a chromatogram and (b) a calibration curve? a. x: time, y...

BACE EXAM 4.0 LABORATORY SKILLS 100% CORRECT the process of cleaning something, especially with a chemical, in order to destroy bacteria. Disinfection Decontamination is the removal of dangerous substances, radioactivity or germs from...? an area object or person Decontamination is the removal of what? dangerous substances radioactivity and germs What is the purpose of aseptic technique? Aseptic technique assures that you don't introduce contaminants into a specimen and that infectious agen...

ASCP MLS Module Exam 2024 Guide Questions Solved 100% Correct A patient has increased serum phosphorus, decreased plasma free calcium, and decreased urinary phosphorus. This correlates with what? Primary hypoparathyroidism For therapeutic drug monitory, how should the specimen be handled? Mixed well to distribute heparin throughout before centrifugation reverse phase high-performance liquid chromatography for therapeutic drug monitory, they term reverse phase implies that the column...

Separation by liquid-liquid extraction is based on a) compound's dielectric constant in 2 dif solvents b) # of theoretical plates in the separation c) use of phase transfer catalysts d) difference in boiling points of 2 liquids e) distribution of a compound between 2 immiscible phases - e) Distribution of a compound between two immiscible phases. Preparative gas chromatography is often chosen over distillation as a means of separation because gas chromatography a) is relatively inexpen...

Use of chromatography and different types - Chromatography is useful because it enables the separation, isolation, purification, and identification of components of extremely complex mixtures - There are different chromatography techniques: - Paper chromatography - Column chromatography - Thin layer chromatography - The results of chromatographic analysis are called chromatograms - The number of spots or peaks correspond to the number of components in the sample - If only one spot...

What does MgSO4 do when added to solution? correct answers It removes water that is dissolved in the solution Why is it more difficult to remove DMSO from a reaction mixture correct answers It has a very high boiling point (90% sure this is correct, but may be wrong) During recrystallization, what will happen if too much hot solvent is used to dissolve the crude compound? correct answers few or zero crystals will be isolated Why is it important to cover the reaction mixture during stirr...

Which reagent is not an oxidizing agent? a. NaOCl b. KMnO4 c. K2Cr2O7 d. CrO3 e. H2O - H2O Which statement is false about preparing alkyl halide from alcohol with hydrogen halide? a. it is an SN1 reaction b. tertiary alcohols react faster than secondary alcohols c. the intermediate is a carbocation d. alkyl halide is soluble in conc. HCl e. in the reaction, the slowest step is the formation of a carbocation - alkyl halide is soluble in conc. HCl 2‐Chloro‐2‐methylpentane was tr...

CHEM 237 - Organic Chemistry Lab Final TAMU Already Graded A Separation by liquid-liquid extraction is based on a) compound's dielectric constant in 2 dif solvents b) # of theoretical plates in the separation c) use of phase transfer catalysts d) difference in boiling points of 2 liquids e) distribution of a compound between 2 immiscible phases e) Distribution of a compound between two immiscible phases. Preparative gas chromatography is often chosen over distillation as a means of separa...